硫辛酸对烧伤家兔炎症介质和氧化应激的影响

Effect of lipoic acid on inflammatory mediators in burn rabbits during stress period

目的观察硫辛酸对烧伤家兔的血清中肿瘤坏死因子-α(TNF-α)、白细胞介素-8(IL-8)、白细胞介素-6(IL-6)和丙二醛(MDA)含量的干预作用。方法在家兔背部两侧造成2处直径为3 cm的圆形烧伤创面,建立深Ⅱ度烧伤新西兰大耳白兔模型。将模型兔随机分为2组:模型组与实验组,每组10只。模型组给予0. 9%NaCl;实验组给予硫辛酸40 mg·kg^(-1)(用0. 9%NaCl溶解硫辛酸),均灌胃给药,每日1次。待家兔创面愈合后,停用药物。分别于烧伤前和烧伤后第1,3,5,7天从家兔耳静脉取血,用放射免疫法检测血清中TNF-α、IL-8与IL-6的含量;用酶联免疫吸附法测定丙二醛(MDA)含量。结果在给予硫辛酸5 d后,实验组与模型组的TNF-α含量分别为(5. 99±2. 36),(7. 12±1. 95) ng·L^(-1);这2组的IL-8含量分别(121. 92±10. 89),(142. 02±9. 87) ng·L^(-1);这2组的IL-6含量分别为(24. 00±7. 15),(26. 12±3. 11) ng·L^(-1);实验组与模型组比较,以上指标差异均有统计学意义(均P <0. 05)。这2组的MDA分别为(5. 37±2. 60),(9. 16±1. 58) nmol·L^(-1),实验组与模型组比较,差异有统计学意义(P <0. 01)。结论硫辛酸可促进烧伤创面愈合,改善氧化应激反应和炎症状态。

Objective To observe the intervention effect of lipoic acid on the changes of tumor necrosis factor-α（ TNF-α）,interleukins-6（ IL-6）,interleukins-8（ IL-8） and malondialdehyde（ MDA） in burn rabbits. Methods New Zealand white rabbits with deep Ⅱ degree burn model was prepared through 2 round wounds with a diameter of 3 cm were created on both sides of the rabbit＇s back. The rabbits model were randomly divided into two groups： model and experimental groups,each group had 10 rabbits. The rabbits in model group were gevin 0. 9% NaCl;the rabbits in experimental group were gevin 40 mg·kg^-1 lipoic acid（ it dissolved in 0. 9% NaCl）; with intragastric administration,1 times a day,until the wound healing of the rabbit. Before burn and at the time of 3,5,7 d after burn,the contents of TNF-α,IL-8,IL-6 were measured by radioimmunoassay; and MDA content was detected by enzyme linked immunosorbent assay. Results After 5 d of lipoic acid administration,serum TNF-α levels in experimental and model groups were（ 5. 99 ± 2. 36）,（ 7. 12 ± 1. 95） ng · L^-1; the IL-8 levels in the 2 groups were（ 121. 92 ± 10. 89）,（ 142. 02 ± 9. 87） ng ·L^-1; the IL-6 levels in the 2 groups were（ 24. 00 ± 7. 15）,（ 26. 12 ± 3. 11） ng·L^-1;comparison between experimental group and model group,the differenceof the factors had significantly（ all P〈0. 05）. The MDA levels in the 2 groups were（ 5. 37 ± 2. 60）,（ 9. 16 ± 1. 58）nmol·L^-1; comparison between experimental group and model group,the difference had significantly（ P〈0. 01）.Conclusion Lipoic acid obviously promotes the healing of burn by improving oxidative stress and inflammation.