ZPR1基因rs603446位点多态性与河南汉族人群血脂异常遗传易感性的关系

Association of ZPR1 gene rs603446 locus polymorphism with susceptibility of dyslipidemia in Henan Han population

目的:研究河南汉族人群锌指蛋白(ZPR1)rs603446位点多态性与血脂异常的关联。方法:从河南农村队列研究中抽取916例血脂异常患者,根据年龄、性别进行1∶1匹配,选取916例血脂正常者作为对照组;采用2×48位SNPscan^(TM)高通量SNP分型技术获取人群rs603446位点的基因型;条件logistic回归分析位点多态性与血脂异常的关联强度;多因子降维法(MDR)结合叉生分析评估基因与环境的交互作用。结果:血脂异常组和正常组ZPR1rs603446位点3种基因型(CC、CT、TT)分布频率差异有统计学意义(P=0.046)。调整相关环境因素后,ZPR1基因rs603446位点突变基因型CT+TT携带者可降低血脂异常的发生(调整OR=0.695,95%CI=0.553～0.872);交互作用分析结果显示,rs603446位点与吸烟具有相加交互作用(调整OR=0.516,95%CI=0.370～0.663),与体力活动具有相乘交互作用(调整OR=0.873,95%CI=0.819～0.930)。结论:ZPR1基因rs603446多态性与血脂异常存在关联,且吸烟、体力活动对血脂异常的发病具有交互作用。

Aim： To investigate the association of zinc finger（ ZPR1） gene rs603446 locus polymorphism and the susceptibility of dyslipidemia（ DYS） in Henan Han population. Methods： A total of 916 DYS subjects and 916 controls based on 1 ∶ 1 matching were collected from the Henan Rural Cohort study; the single nucleotide polymorphism of ZPR1 gene rs603446 was detected using a custom-by-design 2 × 48-Plex SNPscanTM Kit; conditional logistic regression was performed to evaluate the association between ZPR1 gene polymorphism and DYS; multifactor dimensionality reduction（ MDR） model and crossover analysis were used to evaluate the interaction between gene and environment. Results： Three genotypes（ CC,CT and TT） frequency of ZPR1 gene rs603446 between DYS group and control group had significant difference（ P =0. 046）. The data showed that genotype（ CT ＋ TT） of ZPR1 gene rs603446 locus polymorphism decreased the risk of DYS after adjusting environmental risk factors（ adjusted OR = 0. 695,95% CI： 0. 553-0. 872）. Interaction analysis demonstrated that there was additive interaction between rs603446 and smoking（ adjusted OR = 0. 516,95% CI： 0. 370-0. 663）,and multiplicative interaction between rs603446 and physical activity on the susceptibility of DYS was found（ adjusted OR =0. 873,95% CI： 0. 819-0. 930）. Conclusion： ZPR1 gene rs603446 locus polymorphism is associated with the susceptibility of DYS. The interaction between gene and smoking,gene and physical activity is found on the susceptibility of DYS.