姜黄素对非小细胞肺癌A549细胞增殖凋亡和侵袭能力的影响

Effect of Curcumin on Proliferation,Apoptosis and Invasiveness of A549 Cells of Non-Small Cell Lung Cancer

目的探讨姜黄素对非小细胞肺癌A549细胞增殖凋亡和侵袭能力的影响。方法将非小细胞肺癌A549细胞分为对照组和低、中、高剂量组,分别用不含姜黄素的完全培养基和含有质量浓度为10,20,40μmol/L姜黄素的完全培养基培养细胞48 h。采用四甲基偶氮唑盐(MTT)法检测细胞增殖情况,采用流式细胞分析技术检测细胞凋亡情况,采用Transwell侵袭转移实验检测细胞侵袭能力,采用Western Blotting技术检测细胞P27,Orai1,caspase-3,以及表皮生长因子受体(EGFR)、基质交互分子1(STIM1)、E-钙黏蛋白(E-cadherin)、基质金属蛋白酶-9(MMP-9)的表达情况。结果对照组细胞吸光率显著高于低剂量组,低剂量组显著高于中剂量组,中剂量组显著高于高剂量组(P <0. 05);对照组细胞凋亡率、增殖抑制系数显著低于低剂量组,低剂量组显著低于中剂量组,中剂量组显著低于高剂量组(P <0. 05);对照组细胞P27和caspase-3显著低于低剂量组,低剂量组显著低于中剂量组,中剂量组显著低于高剂量组(P <0. 05);对照组Orai1,EGFR,STIM1显著高于低剂量组,低剂量组显著高于中剂量组,中剂量组显著高于高剂量组(P <0. 05);对照组视野细胞数和MMP-9显著高于低剂量组,低剂量组显著高于中剂量组,中剂量组显著高于高剂量组(P <0. 05);对照组侵袭抑制率和E-cadherin显著低于低剂量组,低剂量组显著低于中剂量组,中剂量组显著低于高剂量组(P <0. 05)。结论姜黄素可通过调节非小细胞肺癌细胞蛋白表达来提高肿瘤细胞凋亡率,降低肿瘤细胞增殖能力和侵袭能力,且与姜黄素呈剂量依赖关系。

Objective To investigate the efi-ct of curcumin on the proliferation, apoptosis and invasion of A549 cells of non- small cell lung cancer cells. Methods A549 cells of non- small cell lung cancer were divided into the control group, low dose group, middle dose group and high dose group, they were cultured in a complete medium without curcumin, or cultured in a complete medium containing 10, 20, 40 μmol/L curcumin for 48 h, respectively. The cell proliferation was detected by MTT, the cell apoptosis was detected by flow cytometry. The invasive ability of cells was detected by Transwell invasion and metastasis assay, and the expression of P27, Orail, caspase- 3, EGFR, STIM1, E- cadherin and MMP- 9 was detected by Western Blotting. Results The cell absorptivity of the control group was significantly higher than that of the low dose group, that of the low dose group was significantly higher than that of the middle dose group, and that of the middle dose group was significantly higher than that of the high dose group（P 〈 0.05）. The apoptosis rate and proliferation inhibition coefficient of the control group were significantly lower than those of the low dose group, those of the low dose group were significantly lower than those of the middle dose group, and those of the middle dose group were significantly lower than those of the high dose group（P 〈 0.05）.The P27, caspase-3 of cells of the control group were significantly lower than those of the low dose group, those of the low dose group were significantly lower than those of the middle dose group, and those of the middle dose group were significantly lower than those of the high dose group（P 〈 0.05）. The Orail, EGFR and STIM1 of the control group were significantly higher than those of the low dose group, those of the low dose group were significantly higher than those of the middle dose group, and those of the middle dose group were significantly higher than those of the high dose group（P 〈 0. 05）. The number of cells under visual field and MMP- 9 of the control group were significantly higher than those of the low dose group, those of the low dose group were significantly higher than those of the middle dose group, and those of the middle dose group were significantly higher than those of the high dose group（P 〈 0.05）. The invasion inhibition rate and E- cadherin of the control group were significantly lower than those of the low dose group, those of the low dose group were significantly lower than those of the middle dose group, and those of the middle dose group were significantly lower than those of the high dose group（ P 〈 0.05）. Conclusion Curcumin can increase the apoptosis rate and decrease the proliferation and invasion ability of tumor cells hy regulating the protein expression of non- small cell lung cancer, and this effect is related to the dose dependence of curcumin.