一种用于早期肺癌筛查的新型自身抗体芯片

An auto-antibody Array for the detection of pre-neoplastic lung cancer

非典型增生腺瘤(Atypical adenomatous hyperplasia,AAH)和鳞状细胞增生(squamous cell dysplasia,SCD)与肺部恶性损伤的发展有相关性,精确诊断AAH和SCD有助于早期确诊肺癌,同时有助于肺癌高危人群的筛查与早期干预。本项目利用免疫反应原理,将噬菌体展示技术与生物淘洗技术相结合,获得可表达肿瘤相关蛋白的噬菌体特异性文库,并将噬菌体点制在生物芯片上,筛选得到了与肺部损伤相关的特异性蛋白,经测序分析鉴定出多种自身抗体为恶性肿瘤相关蛋白。最终,通过数据分析,建立筛选分类器,制成筛查检测芯片,用于筛查AAH与SCD,评估早期肺癌风险,表现出重要的临床诊断应用前景。

Atypical adenomatous hyperplasia （AAH） and squamous cell dysplasia （SCD） are associated with the development of malignant lesions in the lung.Accurate diagnosis of AAH and SCD could facilitate earlier clinical intervention and provide useful information for assessing lung cancer risk in human populations.We use T7 bacteriophage vector and Biopanning to develop the microarray.Microarray slides were tested with an independent double-blinded population and then we achieved many autoantibody biomarkers.Both diagnostic values showed promising sensitivity and specificity in detection of pre-neoplastic lung lesions.Hence,this technology could be a useful non-invasive tool to assess lung cancer risk in human populations.