异甘草酸镁对肥胖合并急性坏死性胰腺炎大鼠肝脏的保护作用

Protective effect of Magnesium Isoglycyrrhizinate on acute necrotic pancreatitis induced liver injury in obesity rats

目的建立肥胖合并急性坏死性胰腺炎(ANP)大鼠模型,观察异甘草酸镁对大鼠肝脏的保护作用。方法 24只肥胖雄性SD大鼠,随机分为4组,分别为对照组(H-control组,n=6)、假手术组(H-SO组,n=6)、ANP组(H-ANP组,n=6)和异甘草酸镁干预组(H-ANPT组,n=6)。H-ANP组和H-ANPT组大鼠通过胰胆管逆行注射牛磺胆酸钠方法建立ANP模型,术后12 h剖杀取材。全自动生化仪测量血清淀粉酶(AMY)、脂肪酶(LIPA)、谷丙转氨酶(ALT)和谷草转氨酶(AST)、血清总胆固醇(TC)和甘油三酯(TG)水平;光镜下观察大鼠胰腺、肝脏病理变化;免疫荧光法检测肝脏MPO、IL-1β水平;免疫组化法检测肝脏Caspase-3和NF-κB水平。结果 (1)与H-SO组比较,H-ANP组胰腺、肝脏组织损伤明显加重(P <0.05);H-ANPT组胰腺、肝脏损伤与H-ANP组相比明显改善(P <0.05)。(2)与H-SO组比较,H-ANP组血清AMY、LIPA、AST及ALT水平较H-SO组明显升高(P <0.05);H-ANPT组上述血清指标与H-ANP组相比明显下降(P <0.05)。(3)与H-ANP组相比,H-ANPT组血清TC和TG水平明显下降(P <0.05)。(4)与H-SO组比较,H-ANP组过氧化物酶染色数量、IL-1β明显增加(P <0.05);H-ANPT组上述指标与H-ANP组相比明显降低(P <0.05)。(5)与H-SO组比较,H-ANP组Caspase-3、NF-κB明显增加(P <0.05);H-ANPT组上述指标与HANP组相比明显降低(P <0.05)。结论异甘草酸镁对肥胖大鼠ANP时肝脏损伤具有保护作用。

objective To establish a model of obesity rat combined with acute necrotizing pancreatitis （ANP） and to observe the protective effect of Magnesium Isoglycyrrhizinate on liver. Methods Twenty-four SPF adult male Sprague-Dawley rats were randomly divided into four groups： control group （H-control group, n=6）, shame operation group （H-SO group, n=6）, ANP group （H-ANP group, n=6） and Magnesium Isoglycyrrhizinate group （H-ANPT group, n=6）. ANP model was induced by retrograde infusion of 5% Sodium Taurocholate saline solution into biliopancreatic duct, and rats in each group were sacrifced at 12 hours after the operation. The levels of serum amylase （AMY）, lipase （LIPA）, alanine aminotransferase （ALT） and aspartate aminotransferase （AST） were measured with automatic biochemistry analyzer. Pathological changes of pancreas and liver documents were examined and pathological scores were recorded. Results （1）Compared with the H-SO group, the damages of the pancreas and liver tissues were signifcantly worse in the H-ANP group, and the above damages in the H-ANPT group were signifcantly decreased when comparing with the H-ANP group. （2）Compared with the H-SO group,the serum activities of AMY and LIPA, the levels of AST and ALT were signifcantly increased in the H-ANP group （P〈0.05）, and the above serum indexes were signifcantly lower than those in the H-ANPT group （P〈0.05）. （3）Compared with the H-ANP group, the levels of TC and TG were significantly decreased in the H-ANPT group （P〈0.05）. （4）Compared with the H-SO group, the number of MPO and IL-1β were signifcantly increased in the H-ANP group （P〈0.05）; and the number of MPO and IL-1β in H-ANPT group were signifcantly lower than those in H-ANP group （P〈0.05）. （5）Compared with the H-SO group, the number of Caspase-3 and NF-κB were signifcantly increased in the H-ANP group （P〈0.05）; and the number of Caspase-3 and NF-κB in H-ANPT were significantly lower than those in H-ANP group （P〈0.05）. Conclusion Magnesium Isoglycyrrhizinate may mitigate severe acute pancreatitis-induced liver injuries in obesity rats, which ameliorates liver injury and improves liver function.