摘要
目的建立HPLC法测定甘地胶囊中马钱苷的含量。方法色谱柱为Waters Symmtry C_(18)柱(4.6mm×150mm,5μm);流动相为乙腈-水(13∶87);流速1.0ml/min;检测波长240nm。结果马钱苷对照品在0.030 1～3.01μg范围内呈良好的线性关系(r=0.999 9),平均加样回收率为99.62%,RSD为0.86%。结论本方法简便、准确,重复性好,可用于甘地胶囊中马钱苷的含量测定。
Objective To establish a HPLC method for the assay of loganin in Gandi capsules.Methods The Waters Symmtry column C18（4.6 mm×150 mm,5μm）was used with acetonitrile-water（13∶87）as mobile phase.The flow rate was1.0 ml/min and the detection wavelength at 240 nm.Results The calibration curves were linear within the range of 0.030 1-3.01μg（r=0.999 9）for loganin.The average recovery and relative standard deviation of loganin were 99.62% and 0.86%.Conclusion This method is simple,practicable,accurate and fast.It can be used to assay loganin in Gandi capsules.
作者
魏昕
徐人杰
唐英
姚懁
徐阿晶
张健
WEI Xin1,XU Renjie1, TANG Ying2, YAO Huan2,XU Ajing1, ZHANG Jian1(1 .Department of Pharmacy , Xinhua Hospital Affiliated to School of Medicine, Shanghai Jiao Tong University Shanghai 200092 China;2.Shanghai Fangxin Pharmaceutical Co. , Ltd. Shanghai 200120 ,China)
出处
《药学实践杂志》
CAS
2018年第6期551-553,共3页
Journal of Pharmaceutical Practice
基金
上海市科委项目(15401901600
16401900500)
