NuSAP1在胃腺癌中的表达及临床病理意义

The expression of NuSAP1 in gastric adenocarcinoma and its clinicopathological significance

目的:探讨核仁纺缍体相关蛋白1(nucleolar spindle associated protein 1, NuSAP1)在人胃腺癌(gastric adenocarcinoma, GA)中的表达及与临床病理参数、患者预后的相关性。方法 :应用免疫组织化学方法检测88例GA及癌旁组织中NuSAP1蛋白的表达和定位,分析NuSAP1表达与GA临床病理指标、Ki-67细胞增殖指数及与预后的相关性。结果:NuSAP1蛋白阳性信号定位于癌细胞核。88例GA组织中NuSAP1蛋白低表达43例,高表达45例,NuSAP1蛋白在GA组织中的表达显著高于癌旁组织(P<0.001);NuSAP1蛋白表达与GA组织分化差(P=0.005),TNM分期高(P<0.001),Ki-67增殖指数(P<0.001)呈正相关。Kaplan-Meier生存曲线显示:NuSAP1高表达组患者总生存期显著低于低表达组(P=0.001)。结论:GA组织中NuSAP1高表达可能影响GA增殖、侵袭等过程,GA患者组织中检测NuSAP1的表达可能有提示预后的价值。

Objective： To investigate the expression of nucleolar spindle associated protein I（NuSAP1） in human gastric adenocarcinoma（GA） and the correlation with clinicopathological parameters and prognosis. Methods： Immunohistochemical method was used to detect the expression and localization of NuSAP1 protein in 88 cases of GA and paracancerous tissues. The correlation between NuSAP1 expression and clinicopathological parameters of GA, Ki-67 cell proliferation index and prognosis was analyzed. Results： The positive signal of NuSAP1 protein was located in the nucleus. In 88 cases of GA, NuSAP1 protein was low in 43 cases and high in 45 cases. The expression of NuSAP1 protein in GA tissues was significantly higher than that in adjacent tissues（P〈0.001）. The expression of NuSAP1 protein was positively correlated with GA tissue dif- ferentiation（P=0.005）, TNM stage（P〈0.001） and Ki-67 proliferation index（P〈0.001）. Kaplan-Meier survival curve showed that the overal survival time of the high expression group was lower than that of the low expression group（P=0.001）. Conclusion： The high expression of NuSAP1 in GA tissues may affect the proliferation and invasion of GA. Detection of NuSAP1 expres- sion in GA tissues may be of prognostic value.