摘要
目的研究肉苁蓉枸杞咀嚼片的质量控制方法。方法采用薄层色谱(TLC)法对肉苁蓉枸杞咀嚼片中的肉苁蓉、枸杞子进行定性鉴别;采用高效液相色谱(HPLC)法对肉苁蓉主要成分松果菊苷、毛蕊花糖苷进行定量分析,色谱柱为Hypersil BDS C18(250.0 mm×4.6 mm,5μm),流动相为甲醇-0.1%甲酸溶液,流速1 mL/min,检测波长330 nm,柱温25℃,进样量20μL。结果肉苁蓉和枸杞子的TLC特征斑点清晰、专属性强;松果菊苷和毛蕊花糖苷分别在41.8~418.0μg/mL(r=0.999 9)和40~400μg/mL(r=0.999 9)范围内线性关系良好,回收率分别为99.58%(RSD=0.85%)、99.31%(RSD=1.13%)。结论所建立的定性和定量分析方法能有效控制该咀嚼片的质量。
Objective To establish the quality control methods of Roucongrong Gouqi Jujuepian.Methods TLC was used to identify Roucongrong (Cistanches Herba) and Gouqizi( Lycii Fructus).HPLC was used to deter- mine the contents of echinacoside and verbascoside.The HPLC analysis was performed using a Hypersil BDS C18 (250.0 mm×4.6 mm,5 μm) column at 25 ℃.An isocratic elution with methanol and 0.1% formic acid was used at a flow rate of 1 mL/min. An aliquot of 20 μL of sample solution was injected onto the column and the detection wavelength was 330 nm. Results TLC spots features of Roucongrong ( Cistanches Herba) and Gouqizi ( Lycii Frue- tus) were clear with strong specificity.The linear ranges of echinacoside and verbascoside were 41.8 to 418.0 μg/ mL (r=0.999 9) and 40 to 400 μg/mL (r= 0.999 9).The average sample recoveries were 99.58% (RSD = 0.85% ) and 99.31% ( RSD = 1.13% ). Conclusion The methods reported in this paper can be used scientifically and effectivelly to evaluate the quality of Roucongrong Gouqi Jujuepian.
作者
蒲红利
周博
魏舒畅
PU Hongli;ZHOU Bo;WEI Shuchang(School of Pharmacy,Gansu University of Chinese Medicine,Lanzhou,Gansu,730000,China)
出处
《甘肃中医药大学学报》
2018年第5期41-45,共5页
Journal of Gansu University of Chinese Medicine