传统超声与超声二维斑点追踪技术对小鼠心肌梗死模型评价的比较

Comparison of Traditional Ultrasound with Speckle Tracking Imaging Technique by Myocardial Infarction Model in Mice

目的通过传统超声与超声二维斑点追踪(STI)技术用于小鼠心肌梗死(MI)模型评估的不同特点进行分析比较,确定更精确和准确的技术检测手段。方法 16周龄雄性C57BL/6小鼠随机分为对照组和MI模型组。采用相同参数设置的超声采集对照组小鼠和MI小鼠的心功能情况。运用传统超声方法记录小鼠左心室射血分数(EF%)和缩短分数(FS%),同时利用STI技术分析左室各节段在长轴和短轴上的运动速度、运动位移、应变和应变率4种参数。分析对比传统超声与STI技术对小鼠心功能的评价效果。结果传统超声EF%与FS%能够反映出小鼠发生MI情况,而STI技术不仅能反映小鼠MI情况,还具有模拟心肌各节段运动能力和力学属性的特点,比传统超声更为敏感,反映出的信息更多。结论 STI技术可作为传统超声方法的补充手段,可使小鼠MI模型获得更有效评估。

Objective By analyzing and comparing conventional echocardiography （ECG） to speckle tracking imaging（STI） technique on mice myocardial infarction（MI） model, a more precise in vivo monitoring method is able to be demonstrated. Methods The left anterior descending artery （LAD） ligation （MI group） or sham operation （Sham group） were randomly performed in male C57BL/6 mice at 16 weeks age. Same ultrasound parameters were performed on both groups. Followed conventional ECG method to record ejection fraction （EF%） and fraction shortening （FS%） from left ventricle and utilized STI technique to analyze cardiac cycle velocity, displacement, strain and strain rate of each segment in both short axe and long axe. Compared the results with conventional ECG and STI technique.Results Conventional ECG is able to point out MI status in mice, but STI technique can give more information related to myocardial motor ability and mechanics properties. Thus, it’s more sensitive and informative than conventional ECG. Conclusion STI technique can be used as a supplementary method for MI monitoring in mice that will improve the precision of evaluation.