蛋白质化学修饰的研究进展

Progress on Chemical Modification of Protein

蛋白质是生命的物质基础,其特定的空间结构决定了蛋白质的功能。用化学修饰的方法改变蛋白质结构和功能一直是蛋白质工程改造方面研究的热点。在越来越多的研究报道中,不仅有利用一系列酶对翻译后的蛋白质进行修饰的方法,还有利用小分子物质缀合的修饰方法。该文在分析和总结现有相关文献的基础上,综述了蛋白质末端修饰、侧链上的巯基修饰、二硫键修饰及磷酸化修饰的方法。

Protein is the basic substance of life and its specific spatial structures were very important for the function. Changing the structure and function of proteins by using chemical modification is always the focus in the study of protein engineering. In some reports, many enzymes and chemical modifiers have been used to modify and convert the characteristics of proteins. The former method altered the protein post-translated and the latter conjugat- ed small molecules to protein. Therefor, on the basis of analyzing and summarizing the existing related literatures, we present the methods about chemical modification of protein, including terminal modification, side-chain modifi- cation about thiol, disulfide bond and phosphorylation.