成人隐源性癫痫患者外周血调节性T细胞比值变化的比较

Changes of regulatory T cells in peripheral blood of adults with cryptogenic epilepsy

目的:研讨隐源性癫痫患者外周血CD4^+CD25^+FOXP3^+调节性T细胞(Treg细胞)比值的变化情况,确定隐源性癫痫患者是否存在免疫功能的紊乱。方法:采集28例隐源性癫痫患者和同时期20例健康成人的外周血,使用流式细胞术检测外周血Treg细胞占CD4^+T细胞的比值,及CD4^+T细胞、CD8^+T细胞分别占淋巴细胞的比值。结果:隐源性癫痫组的Treg细胞比例,CD8^+T细胞比例,CD4^+/CD8^+比值分别为(1. 23±0. 31)%,(25. 68±5. 99)%,(1. 47±0. 50)%,对照组的Treg细胞比例,CD8^+T细胞比例,CD4^+/CD8^+比值分别为(1. 56±0. 20)%,(21. 05±3. 90)%,(1. 90±0. 43)%,2组比较差异有统计学意义(P <0. 05)。结论:隐源性癫痫患者体内存在免疫功能的异常,提示免疫机制可能参与到隐源性癫痫的发病中。

Objective ：To study the percentage changes of CD4＋ CD25 ＋ FOXP3＋ regulatory- T cells （ Treg cells ） in peripheral blood of cry-ptogenic epileptic patients, and to determine whether there are disorders of immune function in cry-ptogenic epileptic patients. Methods ：28 patients and 20 healthy adults were enrolled. The percentage of C4＋ CD25＋ FOXP3＋Tregs in CD4＋ T cells and the percentages of C4＋ T cells, CD8 ＋ T cells in lymphocytes were evaluated by flow cytometry. Results ： The percentages of Treg cells, CD8 ＋T cells and C4＋ cells/CD8 ＋ cells in the cry-ptogenic epilepsy group were （ 1.23 ± 0.31 ） %, （25.68 ± 5.99 ） %, and （ 1.47 ± 0.50） % respectively, while the corresponding percentages in the control group were （ 1.56 ± 0.20 ） %, （ 21.05 ± 3.90 ） %, and （ 1.90 ±0.43 ） % respectively. The difference between the two groups was significant（ P 〈 0.05 ）. Conclusion： There is abnormal immune function in patients with cry-ptogenic epilepsy, which suggests that the immune mechanism may be involved in the pathogenesis of cry-ptogenic epilepsy.