β-胡萝卜素-15,15’-加氧酶基因rs11646692位点单核苷酸多态性与血液β-胡萝卜素水平的相关性研究

ASSOCIATION BETWEEN RS11646692 SINGLE NUCLEOTIDE POLYMORPHISM OF β-CAROTENE-15,15'-OXYGENASE(BCO1) GENE AND PLASMA β-CAROTENE LEVELS

目的分析β-胡萝卜素-15,15’-加氧酶(BCO1)基因rs11646692位点单核苷酸多态性(SNP)与血液β-胡萝卜素水平的关系。方法从浙江省宁波市某区体检人群中筛选研究对象(男性94人,女性96人),连接酶检测法检测rs11646692位点基因型,等度反向HPLC法测定血浆β-胡萝卜素水平。结果 t检验显示rs11646692位点基因型的分布在性别间无统计学差异(P> 0.05),女性血液β-胡萝卜素浓度高于男性(P <0.001)。控制性别因素后,广义线性模型分析结果显示rs11646692基因型(P=0.039)、蔬菜摄入量(P=0.037)、以及二者的交互作用(P=0.005)对β-胡萝卜素水平有影响;在蔬菜摄入量大于150g/d的人群中,rs11646692位点为GG型者的β-胡萝卜素浓度高于CC或CG型者(P分别为0.048和0.003)。结论 BCO1 rs11646692 SNP基因型和蔬菜摄入可共同影响血液β-胡萝卜素水平。

Objective To analyze the association between rs11646692 single nucleotide polymorphism （SNP） of β-carotene-15,15＇-oxygenase （BCO1） gene and plasma concentration of β-carotene. Methods 94 males and 96 females were recruited from those participating routine health examination in communities in Ningbo city, Zhejiang province. Genotypes of rs11646692 were detected with ligase detection reaction methods, and plasma β-carotene levels were measured using HPLC. Results Results of t test suggested that there was no significant difference in the distribution of rs11646692 genotypes between genders （P 〉 0.05）, and females had significantly higher levels of plasma β-carotene levels than males （P〈0.001）. After adjustment for gender, general linear model analysis showed that rs11646692 genotypes （P=0.039）, vegetable intake （P=0.037） and the interaction of both factors （P=0.005） were significantly associated with plasma β-carotene levels. In those consuming more than 150 g/day of vegetables, GG carriers had higher plasma t-carotene levels than CC carriers （P=0.048） and CG carriers （P=0.005）. Conclusion The interaction of BCO1 rs11646692 SNP and vegetable intake levels might affect plasma β-carotene levels.