纳米三羟异黄酮Caco-2细胞吸收实验

Experiment on Caco-2 cell uptake of genistein nanoparticles

文章通过实验研究了牛血清白蛋白(bovine serum albumin,BSA)包裹大豆三羟异黄酮(genistein,Gen)纳米体在小肠吸收的可行性。构建Caco-2单细胞模拟小肠上皮细胞对Gen纳米体的吸收转移模型,利用苯酚红通透性试验以及碱性磷酸酶(alkaline phosphatase,AKP)活性检测验证模型,实验结果显示Gen纳米体在跨膜转运的过程中发生了代谢与转化,Gen纳米体在单层细胞模型的顶(apical,AP)侧与基底(basolateral,BL)侧的表观渗透系数Papp均高于Gen单体对照组,说明由BSA包裹的Gen纳米体比Gen单体较易被Caco-2细胞吸收。

Experimental study on the feasibility of absorption of bovine serum albumin（BSA） encapsulaved soybean genistein（Gen） nanoparticles by small intestine was carried out. Caco-2 single cells were used to simulate the uptake and translocation of Gen nanoparticles by small intestinal epithelial ceils, and the phenol red permeation test and the alkaline phosphatase（AKP） activity detection were used to validate the model. The results showed that the Gen nanoparticles occurred with metabolism and transformation in the process of transmembrane transport, and the apparent permeability coefficients （Papp） of the Gen nanoparticles on the apical（AP） side and the basolateral（BL） side of the monolayer cell model were both higher than those of the Gen monomers. It is suggested that the BSA encapsulated Gen nanoparticles are easier to be absorbed by Caco-2 ceils than Gen monomers.