缺血性视神经病变中TREMs的表达

The expression of TREMs in ischemic optic neuropathy of rats

目的探讨大鼠缺血性视神经病变中髓样细胞触发受体（TREMs）的表达及其相关通路。方法模型组采用永久性结扎大鼠双侧颈内动脉14d建立亚急性缺血性视神经病变模型。对照组只分离双侧颈总动脉，不进行结扎。采用逆转录聚合酶链式反应（RT-PCR）和蛋白质印迹法（Western Blot）分别检测髓样细胞触发受体1（TREM-1）、髓样细胞触发受体2（TREM-2）、半胱氨酸天冬氨酸蛋白水解酶3（Caspase-3）、白细胞介素6（IL-6）基因和蛋白表达量。结果模型组TREM-1、Caspase．3、IL-6表达相比对照组明显较高，TREM4表达明显较低，差异均有统计学意义（P=0．001）。结论TREM．1具有促进炎症反应及促凋亡作用，TREM-2作用与TREM-1作用相反。

Objective To investigate the expression of TREMs and the related pathway in ischemic optic neuropathy （ION） of rats. Methods The permanent ligation of bilateral internal carotid arteries （BICA） of rat was performed for 14 days to establish sub-acute ION model as model group. The control group were separated BICA without ligation. The expression of TREM-1, TREM-2, Caspase-3 and IL-6 genes and proteins were detected by reverse transcription PCR （RT-PCR） and Western Blot, respectively. Results Compared with control group, the expressions of TREM-1, Caspase-3 and IL-6 in model group were higher and TREM-2 was lower （P = 0. 001 ）. Conclusion TREM-1 has effects on inducing apoptosis and inflammation. Reversely, TREM-2 shows opposite effects.