AMPK激活对血管损伤内皮修复相关因子的影响

Effect of AMPK Activation on the Related Factors of Vascular Injury Repair

为探究AMPK激活对血管损伤内皮修复相关因子的影响，本研究采用流式细胞技术和免疫荧光技术，获得外周血表达干细胞标记CD133（91．50／0），CD34（91．5％），及表达内皮细胞表面抗原CD31（91．9％），VEGFR-2（91．7％）的内皮祖细胞，通过固相芯片技术检测EPCs分泌40种生长因子的变化情况，试验分为6组：对照组、LPS处理组、AICAR处理组、LPS处理组＋AICAR处理组、AS-605240处理组、LPS处理组＋AS-605240处理组。研究显示AMPK激动剂AICAR能促进EPCs分泌不同类型生长因子，而AMPK抑制剂AS-605240则明显抑制EPCs分泌生长因子。本研究提示AMPK对人外周血EPCs分泌生长因子有重要的影响，能激活EPCs分泌多种生长因子，进而促进血管损伤后内皮的修复。

To investigate the effect AMPK activation on endothelial repair related factors in vascular injury, the flow cytometry and immunofluorescence technique were used in this study to obtain the stem cell marker CD133 （91.5%）, CD34 （91.5%） in peripheral blood, and the endothelial progenitor cells which express endothelial cell lineage antigen CD31 （91.9%） and VEGFR-2 （91.7%）. The changes of 40 kinds of growth factors secreted by EPCs were detected by solid phase chip technique. The experiments were divided into 6 groups： control group, LPS group, AICAR treatment group, LPS and AICAR treatment group, AS-605240 treatment group, LPS and AS-605240 treatment group. The results showed that AICAR, a AMPK agonist, could promote EPCs to produce multiple growth factors, while AS-605240, a AMPK inhibitor, could decreased cytokines release. It could be indicated that AMPK has important effects on the secretion of growth factors in human peripheral blood, it coule activate EPCs to secrete many growth factors, and then promote endothelial repair after vascular injury.