调脾承气汤对实验动物胃肠功能的影响

Effect of Tiaopi Chengqi decoction on gastrointestinal function of experimental animals

目的:观察调脾承气汤对实验动物胃肠功能的影响。方法:测定食积模型小鼠胃排空及小肠推进率;采用放射免疫分析法检测小鼠血清中胃泌素(GAS)、胃动素(MTL)、生长抑素(SS)、血管活性肠肽(VIP)的含量;运用滴定法及麦特(Mett)改良法检测大鼠胃酸含量及胃蛋白酶活性。结果:调脾承气汤高、中剂量可提高小鼠小肠推进率,加快胃的排空。治疗组小鼠血清MTL、SS含量增加,GAS、VIP含量减少,与模型组比较差异有统计学意义(P<0.05,P<0.01)。调脾承气汤具有提高大鼠胃蛋白酶活力的作用,但对胃酸分泌影响不大(P>0.05)。结论:调脾承气汤治疗食积的作用机制可能与其调节胃肠激素有关。

Objective：To observe the effect of Tiaopi Chengqi decoction on gastrointestinal function of experimental animals.Methods：After the models were established,gastric emptying and intestinal propulsive rate,the levels of MTL,SS,GAS and VIP of model mice were determined. Gastric acid and pepsin activity of model rats were determined. Results：Compared with the model group,the levels of MTL and SS were increased,and the contents of GAS and VIP in serum of mice in treatment groups were decreased（P〈0.05,P〈 0.01）. Pepsin activity of rats in treatment groups was increased（P〈 0.05）.Conclusion：The mechanism of Tiaopi Chengqi decoction in treating food accumulation models may be related to its regulation on gastrointestinal hormones.