富含血小板的白膜在保存期内中性粒细胞功能的变化

The function change of the neutrophilic granulocytes during the preservation period

目的探讨在24 h内,随着时间的变化,富含血小板的白膜内的保存状态对中性粒细胞（PMN）功能的影响。方法随机抽取10袋400 mL健康成人全血采集6 h内分离出富含血小板的白膜,每袋白膜（40—60 mL）平均3份,分别按采集时间6 h、12 h、18 h分组,放置在22℃温箱保存。用流式细胞仪分选PMN并测定吞噬、活性氧的分泌和凋亡功能。结果 1）吞噬功能：6 h组和12 h组比较没有统计学意义（Ρ〉0. 05）,与6 h组比较,12 h组、18 h都有统计学意义的下降（Ρ〈0. 05）。2）活性氧：6 h组、12 h组、18 h组两两比较没有统计学意义（Ρ〉0. 05）。3）凋亡（凋亡和坏死）：12 h组和6 h组比没有统计学意义（Ρ〉0. 05）,18 h组和12 h组比较也没有统计学意义（Ρ〉0. 05）,6 h组和18 h组比较有统计学意义的上升（Ρ〈0. 05）。结论中性粒细胞的吞噬功能和凋亡都在12 h后开始出现变化,采集时间在12 h后的白膜制品应尽早输注。吞噬功能可以更敏感的反应PMN的功能变化。

Objective To investigate the effect of the preservation status of the storage film containing platelets on the function of the neutrophil（ PMN） in 24 h. Methods random 10 bags of 2 qualified blood units（ 400 mL） were collected and platelet-rich white film was isolated within 6 h,each bag of 40—60 mL film. Each of the film was further divided into 3 groups and stored at 22℃ in boxes for 6 h,12 h and 18 h respectively. Neutrophils were then isolated by flow cytometry.Phagocytosis,active oxygen secretion and apoptosis were measured. Results the phagocytic function of the 6 h and 12 h group had no significant difference（ Ρ0. 05）; The 6 h,12 h and 18 h group had significant differences in the activity of oxygen（ Ρ0. 05）; The 6 h group,12 h group,18 h group of 22℃ showed no significant difference. Apoptosis（ apoptosis and necrosis） ： The 12 h group and 6 h group compared with no significant difference（ Ρ0. 05）,18 h group,12 h group has no significant difference（ Ρ0. 05）,6 h group,18 h group had significant differences（ P〈0. 05）. Conclusion the phagocytic function and apoptosis of neutrophilic granulocytes began to change at 12 h. The neutrophilic products should be injected as soon as possible afterwithin athe collection time of 12 h. Phagocytic function can be more sensitive to the functional changes of PMN.