少数民族中小学生B族维生素关联基因多态性地域分布特征

Regional feature in vitamin B12 related gene polymorphisms among minority students in primary and middle schools

目的研究中国少数民族在校学生B族维生素缺乏风险的关联基因多态性(SNP)位点地域特征,为基因多态与在校学生人群营养素缺乏风险关系研究提供基础资料。方法搜集以往研究中报道的25个SNP位点,采用2016年广西、内蒙和新疆地区384名贫困农村学生的凝固血细胞,用磁珠法提取DNA,Sequenom MassArray飞行质谱检测系统进行基因分型检测,采用SPSS 16.0软件对数据结果进行分析。结果11个SNP位点分型结果在地域分布上差异存在统计学意义(P值均<0.05)。19号染色体上rs492602和rs602662进行连锁分析结果显示,3个地区均出现强连锁不平衡,r^2值均为1。21号染色体上rs234706和rs234713进行连锁分析的结果显示,3个地区均显示出强连锁不平衡,广西地区r^2值为0.81。结论3个少数民族在校学生的B族维生素营养状况关联基因多态性存在区域差异。

Objective To analyze the regional feature vitamin B12-related gene polymorphisms in minority students,and to provide basic data for the relevant study.Methods This study collected 25 SNPs of the previous reports and the blood cell samples of 384 minority adolescents in the poor rural areas from Guangxi,Inner Mongolia and Xinjiang in 2016,DNA was extracted with immunomagnetic beads,Sequenom MassArray genotyping assay,and data results were analyzed by SPSS 16.0.Results The results showed that 11 SNPs were of statistically significant differences in the geographical distribution.The r^2 were 1 between rs492602 and rs602662 on chromosome 19 for Uygur,Mongolian and Zhuang.The r^2 between rs234706 and rs234713 on chromosome 21 was found imbalanced in the three areas.The r^2 was 0.81 for students in Guangxi.Conclusion The study showed regional differences in vitamin B12 gene polymorphisms among minority adolescents.