TrkA、Ki-67、CD34在肾透明细胞癌中的表达及临床意义

Expressions and significances of TrkA,CD34 and Ki-67 in renal clear cell carcinoma

目的探讨神经生长因子受体络氨酸受体激酶A(TrkA)和CD34及Ki-67在肾透明细胞癌中的表达及临床意义。方法应用免疫组化方法检测48例肾透明细胞癌组织、25例正常肾脏组织中TrkA、CD34及Ki-67的表达。应用SPSS 16. 0统计软件,不同组间TrkA、CD34及Ki-67的阳性表达的比较采用交叉表χ~2检验。TrkA与CD34及Ki-67的表达的相关性用Spearman等级相关分析。结果肾透明细胞癌组和正常肾组织组中:TrkA的表达阳性率较高(83. 0%和58. 3%),两组间比较差异有统计学意义(χ~2=5. 416,P=0. 026); CD34在肾透明细胞癌组中的表达阳性率与正常肾组织组相比明显增高(39. 6. 0%和4. 2%),差异有统计学意义(χ~2=27. 422,P <0. 01); Ki-67在肾透明细胞癌组中的阳性表达率高于正常肾脏组(24. 5%和0),二者比较差异有统计学意义(χ~2=7. 083,P <0. 01)。在转移瘤及高分期肿瘤中TrkA的阳性表达与CD34及Ki-67的阳性表达具有相关性(r=0. 911、0. 548、0. 588、0. 594)。结论 TrkA在肾透明细胞癌中高表达,在高分期及转移瘤中与CD34及Ki-67的表达有相关性。联合检测TrkA、CD34及Ki-67对肾透明细胞癌诊断和预后可能有一定的临床意义。

Objective To investigate the expression and significance of the nerve growth factor receptor TrkA,CD34 and Ki-67 in renal cell carcinoma. Methods Immunohistochemistry was used to detect the expression of TrkA,CD34 and Ki-67 in 53 cases of renal cell carcinoma and 24 cases of normal kidney tissues. SPSS16. 0 statistical software was applicated to detected the differences of the expression of TrkA,Ki-67 and CD34 in different groups. Spearman rank correlation analysis was used to detect the expression of TrkA,CD34 and Ki-67. Results Compare with normal kidney group,the expression rate of TrkA in renal cell carcinoma was higher（ 83. 0% and 58. 3%）,and the difference was significant（ χ^2=5. 416,P = 0. 026）. The positive rate of CD34 expression in renal clear cell carcinoma group was significantly higher than that in normal renal tissue group（ 39. 6. 0% and 4. 2%）,and the difference was significant（ χ^2= 27. 422,P〈0. 01）; Higher Ki-67 expression was detected in the renal clear cell carcinoma group compared with that in the normal kidney group（ 24. 5% and 0）,there was significant difference between the two groups（ χ^2= 7. 083,P〈0. 01）. The positive expression of TrkA was correlated with CD34 and Ki-67 in the metastatic or high staging tumors（ r = 0. 911,0. 548,0. 588,0. 594）.Conclusions The high expression of TrkA in renal cell carcinoma is correlated with the high expression of CD34 and Ki-67 in the metastatic and high stages tumors. Combined detection of TrkA,CD34 and Ki-67 in renal clear cell carcinoma and may has certain significance for the diagnosis and prognosis.