gga-miR-155对MDCC-MSB1细胞生物学行为的影响

Effect of gga-miR-155 on the Biological Behavior of MDCC-MSB1 Cells

为了解gga-miR-155对MDCC-MSB1细胞生物学行为的影响,将人工合成的gga-miR-155模拟物、gga-miR-155抑制物以及阴性对照瞬时转染MDCC-MSB1细胞。然后采用实时荧光定量PCR(RT-qPCR)检测MDCC-MSB1细胞中gga-miR-155的表达水平,CCK-8法检测转染后细胞增殖能力的变化,流式细胞术检测MDCC-MSB1细胞的细胞周期与凋亡的变化,Transwell检测MDCC-MSB1细胞的迁移、侵袭能力的变化。结果显示,gga-miR-155模拟物可显著上调MDCC-MSB1细胞gga-miR-155表达水平,促进MDCC-MSB1细胞增殖,细胞G1期细胞减少,S和G2期细胞增加,同时凋亡减少,迁移、侵袭能力增加;gga-miR-155抑制物可显著下调MDCCMSB1细胞gga-miR-155表达水平,抑制MDCC-MSB1细胞增殖,细胞G1期细胞增加、S和G2期细胞减少,同时凋亡增加,迁移、侵袭能力下降。结果表明,gga-miR-155可促进MDCC-MSB1细胞增殖、迁移和侵袭,抑制其凋亡。

In order to detect the effect of gga miR 155 on the biological behavior of MDCC MSB1 cells, MDCC MSB1 cells were transiently transfected with gga miR 155 mimic, gga miR 155 inhibitor, and negative control which were artificial synthesized. Then, the expression of gga miR 155 was detected by real time PCR （qRT PCR） ; the cell proliferation was detected by CCK 8 assay; the cell cycle and apoptosis were analyzed by flow cytometry; and the cell migration and invasive ability were determined by transwell assay. Results showed that gga miR 155 mimic upregulated the expression level of gga miR 155 in MDCC MSB1 cells. After gga miR 155 mimictransfection, the proliferation of MDCC MSB1 cells was promoted, G1 phase cells were decreased, S and G2 cells were increased, the apoptosis rate was reduced,the migration invasion capability were increased in gga miR 155 mimic transfected cells. On the contrary, the gga miR 155inhibitor significantly down regulated the expression level of gga miR 155 in MDCC MSB1 cells.When the gga miR 155 inhibitor was transfected into MDCC MSB1 cells, cell proliferation wasinhibited,G1 phase cells were increased, S and G2 cells were reduced, the cell apoptosis was increased, and the migration invasion capability were suppressed. The results showed that ggamiR 155 could promote the proliferation, migration and invasion of MDCC MSB1 cells and inhibittheir apoptosis.