刺老苞根皮醇提物对原代成骨细胞增殖、分化和矿化的影响

Efficacy of Aralia Echinocaulis on Proliferation, Differentiation and Mineralization of Primary Osteoblast

目的:研究4种不同浓度刺老苞根皮醇提物对原代成骨细胞的增殖、分化及矿化的影响。方法:通过消化10只雄性新生SD乳鼠(SPF级)的颅盖骨,获得原代前成骨细胞体外培养、传代,10 mmol/Lβ-甘油磷酸钠和50μg/mL抗坏血酸诱导,碱性磷酸酶(ALP)染色和矿化结节染色鉴定原代成骨细胞,分别用低、中、高浓度(1、10、100μg/mL)刺老苞根皮醇提物(4种)对成骨细胞进行干预,培养4 d和14 d后分别进行ALP活性测定和茜素红染色面积测定。结果:与对照组比较4种刺老苞根皮醇提物均能提高成骨细胞生存率,增强ALP活性,增加矿化结节面积。结论:4种刺老苞根皮醇提物可通过促进成骨细胞增殖和分化,加快骨基质矿化过程。

Objectives： The influence of different concentration groups of 4 kinds ethanol extracts from Aralia echinocauIis Hand.-Mazz. on the proliferation, differentiation and mineralization of primary osteoblasts were observed. Methods： The calvarias of 10 male SD neonatal rats were digested to obtain primary preosteoblasts in vitro subculture. The preosteoblasts were induced to osteoblasts by 10 mmol/L β-glycerin phosphate and 50 μg/mL L-ascorbic acid. Alkaline phosphatase（ALP） and mineralized nodule staining were used to identify primary osteoblasts. The osteoblasts were respectively cultured with 4 kinds of alcohol extracts（1, 10, 100 g/mL） from Aralia echinocauIis Hand.-Mazz. After 4 and 14 days, the activity of ALP and alizarin red staining were measured respectively. Results： compared with the control group, the 4 kinds of alcohol extracts from Aralia echinocauIis Hand.-Mazz. could increase the survival rate of osteoblast, the activity of ALP and the mineralized area. Conclusion： 4 kinds of alcohol extracts from Aralia echinocauIis Hand.-Mazz. can promote the proliferation and differentiation of osteoblasts. Then, increasing the mineralization of bone matrix.