杨树花化学成分的分离鉴定Ⅱ

Isolation and Identification of Chemical Constituents of Populus tomentosa Male Inflorescence Ⅱ

目的：杨树花Poprli Flos作为我国传统中药,大量研究发现其含有多种化学成分,且具有良好的抗菌、抗炎等药理作用。该文旨在对中药杨树花乙酸乙酯部位进行系统的化学成分研究,丰富其研究资料,为相关新药研发及临床应用奠定基础。方法：取毛白杨干燥雄花序30 kg,采用70%乙醇回流提取3次,减压回收溶剂得浸膏,分散于适量水中,分别用石油醚、乙酸乙酯、正丁醇萃取。取乙酸乙酯萃取物经硅胶,ODS,LH-20型羟丙基葡聚糖凝胶（Sephadex LH-20）柱色谱、重结晶等方法进行系统分离纯化,结合理化性质并利用ESI-MS,NMR等波谱学技术对所得化合物进行结构鉴定。结果：从乙酸乙酯部位中分离得到12个单体化合物,鉴定结构为槲皮素（1）,花旗松素（2）,木犀草素（3）,圣草酚（4）,杨梅素（5）,二氢杨梅素（6）,（2S）-5,7-二羟基黄烷酮-7-O-β-D-葡萄糖苷（7）,原儿茶酸（8）,咖啡酸（9）,1,3-二咖啡酰基-2-乙酰基甘油（10）,柳皮苷-6＇-苯甲酸酯（11）,胡萝卜苷（12）。结论：化合物2,5-7为首次从杨属植物中分离得到,化合物2,4-8,10,11为首次从该植物中分离得到。该研究为中药杨树花的综合运用提供了一定的化学依据。

Objective： A great deal of researches have shown that Populus tomentosa male inflorescence,as our traditional Chinese medicine, has a variety of chemical constituents and strong anti-bacterial, antiinflammatory and other pharmacological effect. The aim of this study was to investigate the chemical constituents of P. tomentosa systemically to enrich its research materials and lay foundations for the development of new drugs and its clinical application. Method： The male anthotaxy of P. tomentosa male inflorescence（ 30 kg） was extracted for three times with 70% ethanol,concentrated under reduced pressure,and then the extract was suspended in water. Then the solvent was partitioned with petroleum ether,Et OAc and n-Bu OH successively. The components of Et OAc extract were isolated and purified by ODS,silica gel,Sephadex LH-20 column chromatography and recrystallization. Their structures were identified based on the physiochemical properties and spectroscopy techniques such as ESI-MS and nuclear magnetic resonance（ NMR）. Result： Twelve compounds were isolated from the ethyl acetate fraction, and they were identified as quercetin（ 1）, taxifolin（ 2）, luteolin（ 3）,eriodictyol（ 4）, myricetin（ 5）, dihydromyricetin（ 6）,（ 2 S）-5,7-dihydroxyflavanone-7-O-β-D-glucopyranoside（ 7）,protocatechuic acid（ 8）,caffeic acid（ 9）,2-acetyl-1,3-dicaffeoylglycerol（ 10）,salicortin-6＇-benzoate（ 11）,and daucosterol（ 12）,respectively. Conclusion： Compounds 2,5-7 were isolated from the Populus genus for the first time,and Compounds 2,4-8,10,11 were isolated from the plant for the first time,providing certain chemical basis for comprehensive utilization of Populus tomentosa.