摘要
Objective： The leaves of Chromolaena odorata, a highly invasive shrub found growing wild worldwide, are traditionally used for wound healing. Due to its high flavonoid contents, we aimed to find a new appli- cation for this plant. Preliminary tests using its ethanolic leaf extract showed that it could suppress the accumulation of lipids in adipocytes. We therefore studied the anti-adipogenic effect of several C. odorata leaf extracts and the relationship between molecular structure and bio-activity of its isolated flavonoid constituents using 3T3-LI preadipocytes/adipocytes as a model. Methods： Three leaf extracts and thirteen flavonoids isolated from C odorata were tested for their effect on lipid accumulation in 3T3-L1 adipocytes using AdipoRed reagent, with quercetin as the positive con- trol. The effects of active flavonoids on the adipocytes were confirmed by oil red O staining and visualized under a light microscope.Results： n-Hexane and ethyl acetate extracts of C odorata leaves displayed anti-adipogenic activity. The latter extract was the more potent one, especially at 40 μg/mL. Four flavonoids, pectolinarigenin, kaemp- feride, 4,2＇-dihydroxy-4＇,5＇,6＇-trimethoxychalcone and dillenetin, exhibited significant, concentration- dependent inhibitory effects on lipid accumulation in 3T3-L1 adipocytes. The most potent flavonoid obtained in this study was 4,2＇-dihydroxy-4＇,5＇,6＇-trimethoxychalcone, which caused 75% and 90% inhi- bition of cellular lipid accumulation at 30 and 50μmol/L, respectively. Both kaempferide and 4,2＇-dihy droxy-4＇,5＇,6＇-trimethoxychalcone were major constituents in the ethyl acetate extract of this plant.Conclusion： C odorata leaves contained several flavonoids with anti-adipogenic effects against lipid accu- mulation in 3T3-L1 adipocytes. The plant, normally considered a useless weed, may actually provide an abundant source of biologically active flavonoids.
Objective： The leaves of Chromolaena odorata, a highly invasive shrub found growing wild worldwide, are traditionally used for wound healing. Due to its high flavonoid contents, we aimed to find a new appli- cation for this plant. Preliminary tests using its ethanolic leaf extract showed that it could suppress the accumulation of lipids in adipocytes. We therefore studied the anti-adipogenic effect of several C. odorata leaf extracts and the relationship between molecular structure and bio-activity of its isolated flavonoid constituents using 3T3-LI preadipocytes/adipocytes as a model. Methods： Three leaf extracts and thirteen flavonoids isolated from C odorata were tested for their effect on lipid accumulation in 3T3-L1 adipocytes using AdipoRed reagent, with quercetin as the positive con- trol. The effects of active flavonoids on the adipocytes were confirmed by oil red O staining and visualized under a light microscope.Results： n-Hexane and ethyl acetate extracts of C odorata leaves displayed anti-adipogenic activity. The latter extract was the more potent one, especially at 40 μg/mL. Four flavonoids, pectolinarigenin, kaemp- feride, 4,2＇-dihydroxy-4＇,5＇,6＇-trimethoxychalcone and dillenetin, exhibited significant, concentration- dependent inhibitory effects on lipid accumulation in 3T3-L1 adipocytes. The most potent flavonoid obtained in this study was 4,2＇-dihydroxy-4＇,5＇,6＇-trimethoxychalcone, which caused 75% and 90% inhi- bition of cellular lipid accumulation at 30 and 50μmol/L, respectively. Both kaempferide and 4,2＇-dihy droxy-4＇,5＇,6＇-trimethoxychalcone were major constituents in the ethyl acetate extract of this plant.Conclusion： C odorata leaves contained several flavonoids with anti-adipogenic effects against lipid accu- mulation in 3T3-L1 adipocytes. The plant, normally considered a useless weed, may actually provide an abundant source of biologically active flavonoids.
基金
supported by a grant from Thailand Graduate Institute of Science and Technology(TGIST)(grant number:01-54-007)
Nano-Cosmeceuticals Laboratory,National Nan-otechnology Center(NANOTEC)
National Science and Technology Development Agency(NSTDA),Thailand
