骨髓间充质干细胞移植对脑动脉栓塞大鼠VEGF165、Notch1表达的影响

The effect of bone marrow mesenchymal stem cells transplantation on the expression of VEGF165 and Notch1 in rats with cerebral arterial embolization

为探究骨髓间充质干细胞(BMSCs)移植对脑损伤大鼠的改善作用机制,本实验通过建立脑损伤大鼠模型,观察BMSCs移植对脑动脉栓塞大鼠血管内皮生长因子(VEGF) 165、Notch1表达的影响。将45只SD大鼠随机分为假手术对照组、动脉栓塞(MCAO)组、BMSCs移植组。检测脑组织栓塞区微血管数量,及各组大鼠VEGF165、Notch1蛋白和mRNA的表达。结果发现MACO组、BMSCs移植组大鼠的脑组织中微血管数量明显多于假手术对照组; MACO组和BMSCs移植组的VEGF165与Notch1的蛋白表达、VEGF165与Notch1的mRNA表达均高于假手术对照组; BMSCs移植组较MACO组更高。根据结果推测BMSCs移植可能通过激活Notch信号通路促进VEGF165的表达,而促进脑动脉栓塞区的血管新生。

In order to explore the mechanism of bone marrow mesenchymal stem cell （BMSCs） transplantation on improving brain injury in rats, the effect of BMSCs transplantation on the expression of vascular endothelial growth factor （VEGF） 165 and Notch1 in rats with cerebral artery embolization was observed by establishing a rat model of brain injury. 45 SD rats were randomly divided into the sham operation control group, the middle cerebral artery occlusion （MACO） group and the BMSCs transplantation group. The number of microvessel in cerebral embolism area and the expression of VEGF165, Notch1 protein and mRNA in each group were detected.The number of microvessels in the brain tissue of the MACO group and the BMSCs transplantation group was significantly more than that in the sham control group. The protein expression of VEGF165 and Notch1, and the mRNA expression of VEGF165 and Notch1 in the group MACO and the BMSCs transplantation group were higher than those in the sham operation control group. The BMSCs group was higher than the MACO group. The conclusion is that BMSCs transplantation may promote the expression of VEGF165 by activating the Notch signaling pathway. Thus, it can promote the angiogenesis in the cerebral artery embolism area.