干细胞动员对肺损伤大鼠模型肺功能保护及机制研究

Study on lung function protection and mechanism of stem cell mobilization in rat model of lung injury

为了探讨干细胞动员对肺损伤大鼠的肺功能保护及机制研究,将雄性wistar大鼠30只,随机分为正常组、对照组和动员组,每组各10只。观察肺组织病理改变并检测3组大鼠肺泡灌洗液肺组织湿干比和中性粒细胞数,ELISA测定肺组织匀浆肿瘤坏死因子(TNF)-α、白细胞介素(IL)-1、白细胞介素(IL)-6水平。结果显示,干细胞动员可以减轻肺损伤大鼠模型的损伤程度。对照组和动员组的肺泡灌洗液中性粒细胞数均高于正常组,动员组低于对照组。对照组的肺组织湿干比高于正常组,动员组低于对照组。肺组织匀浆中对照组的TNF-α、IL-1、IL-6的表达水平均明显高于正常组,动员组低于对照组。研究结果表明,干细胞动员能有效保护大鼠肺损伤,减少炎性因子释放,促进肺功能恢复。

To investigate the protection and mechanism of lung function in rats with lung injury by stem cell mobilization. 30 male Wistar rats were randomly divided into the normal group, the control group and the mobilization group with 10 rats in each group. The pathological changes of lung tissue were observed. The wet dry ratio and neutrophil count of lung tissue in 3 groups of rats were detected. The levels of tumor necrosis factor （TNF） - alpha, interleukin （IL） -1 and interleukin （IL） -6 in lung homogenate were measured using ELISA kit. The results showed stem cell mobilization can reduce the degree of injury in rat models of lung injury .The number of neutrophils in the alveolar lavage fluid of the control group and the mobilization group were all higher than that of the normal group, and the mobilization group was lower than the control group. The ratio of wet and dry of lung tissue in the control group was higher than that of the normal group, and the mobilization group was lower than that of the control group. In the control group the expression levels of TNF-a, IL-1 and IL-6 in the lung homogenate were significantly higher than those in the normal group, and the mobilized group was lower than that in the control group. These results suggest that stem cell mobilization can effectively protect the lung injury of rats, reduce the release of inflammatory factors and promote the recovery of lung function.