嗜水气单胞菌和温和气单胞菌快速PCR方法检测

Detection of Aeromonas hydrophila and A. sobria by PCR Method

本研究根据GenBank数据库已发表的气单胞菌属16S rDNA序列,设计嗜水气单胞菌、温和气单胞菌16S rDNA序列的特异保守区,建立这2种菌的快速PCR检测方法。结果表明,仅嗜水气单胞菌和温和气单胞菌得到了特异性扩增,而几种常见弧菌科水产病原菌,包括豚鼠气单胞菌、易损气单胞菌、杀鲑气单胞菌、副溶血弧菌、创伤弧菌、溶藻弧菌和鳗弧菌并未得到特异性扩增,说明设计的引物可以用于这2种病原的PCR快速检测方法的建立,可以为水产动物嗜水气单胞菌、温和气单胞菌的快速检测、病害预警和病原调查提供技术支撑。

Based on the 16S rDNA sequence of Aeromonas hydrophila published in GenBank database,the specific conserved region of 16SrDNA sequence of Aeromonas hydrophila and Aeromonas sobria was designed and a rapid PCR method was established for the detection of these twobacteria.The results showed that only Aeromonas hydrophila and Aeromonas sobria were specifically amplified,while several common aquaticpathogens,including Aeromonas hydrophila,Aeromonas caviae,Aeromonas trota,Aeromonas salraonicida Vibrio parahaemolyticus,V. vulnificus,V.anguillarum,V. alginolyticusere were not specifically amplified. Thus the primers could be used for rapid detection of the two pathogens and providedthe technical support for disease warning and pathogen investigation of Aeromonas hydrophila and Aeromonas sobria.