妊娠期糖尿病患者血清miR-222和雌激素受体-α表达的临床意义

The expression of serum MiR-222 and estrogen receptor-α(ERα) of pregnant women with gestational diabetes mellitus and its clinical significance

目的:探讨血清微小miR-222和雌激素受体-α(ERα)在妊娠期糖尿病患者中的表达及其临床意义。方法:收集2014年8月-2016年8月本院治疗并分娩的100例妊娠期糖尿病患者为观察组,另选同期本院产检待分娩的血糖正常孕妇100例为对照组。观察两组孕妇临床指标,qRT-PCR法检测血清miR-222与ERαmRNA表达量,Western blot检测ERα蛋白表达水平,比较miR-222、ERα表达与患者临床指标关系,Pearson法分析miR-222、ERα表达相关性,并根据检测中位值将miR-222、ERα分别分为高表达组与低表达组,分析其与新生儿预后的关系。结果:观察组孕妇BMI、FPG、FINS均高于对照组(P<0.05);与对照组相比,观察组血清miR-222表达量升高,ERαmRNA表达量降低,ERα蛋白表达水平降低(P<0.05);观察组miR-222与BMI、FPG、FINS、TC均呈正相关,ERαmRNA与FPG呈负相关(均P <0.05);Pearson相关性分析miR-222与ERαmRNA表达水平呈负相关(P <0.05);miR-222、ERαmRNA表达均与新生儿病理性黄疸相关(P<0.05)。结论:妊娠期糖尿病患者血清miR-222上调表达调控ERα下调表达,进而参与妊娠期糖尿病发生发展进程,二者表达呈负相关。提示可能作为临床孕早期诊断妊娠期糖尿病及评估新生儿预后的生物学指标。

Objective To investigate the expression of serum MiR 222 and estrogen receptor α （ERα） of pregnant women with gestational diabetes mellitus （GDM）, and to analyze its clinical significance. Methods： Collected 100 pregnant women with GDM in affiliated hospital of north China Science and Technology University were selected into research group from August 2014 to August 2016, and another 100 normal pregnant women during the same period were selected into control group. The clinical indexes of all included pregnant women in the two groups were observed. qRT PCR method was used to detect the expressions of serum miR 222 and ER mRNA, Western blot was used to detect the expression level of ERαprotein. the relationship between the expressions of miR 222 or ERαand the clinical indicators of pregnant women with GDM were compared between the two groups. Pearson method was used for further analyzing the correlation between the expression of miR 222 and the expression level of ERα. And according to the test results of the median value of miR 222 and ERαexpression, pregnant women with GDM were divided into high expression group and low expression group, and the relationships between miR 222 or ERαexpression and the prognosis of the newborns were analyzed. Results： The BMI, FPG and FINS of pregnant women in research group were significant higher than those of pregnant women in control group （ P 〈0.05）. Compared with the control group, the expression of serum miR 222 of pregnant women in research group was increased significantly （ P 〈0.05）, while the expression of ERαmRNA and the level of ER protein expression were decreased significantly （ P 〈0.05）. The expression of miR 222 of pregnant women with GDM was positively correlated with BMI, FPG, FINS or TC （ P 〈0.05）, while the expression of ERαmRNA was negatively correlated with FPG （ P 〈0.05）. Pearson correlation analysis had showed that the expression of miR 222 was negatively correlated with ER mRNA expression （ P 〈0.05）, and the expressions of miR 222 or ERαmRNA were significant correlated with neonatal pathological jaundice （ P 〈0.05）. Conclusion： The up regulation of serum miR 222 expression of pregnant women with GDM can regulate down the expression of ER, and then involve in the occurrence and development of GDM. As for pregnant women with GDM, serum miR 222 expression is negatively correlated with ER mRNA expression, so it can be used as a biological indicator for early diagnosing GDM of pregnant women, and also can be as a biological indicator for evaluating neonatal prognosis.