促红细胞生成素对缺血心肌细胞LC3-Ⅱ和P62的影响

Effect of erythropoietin( EPO) on autophagy of ischemia myocardial cells

目的:探讨促红细胞生成素(EPO)对缺血心肌细胞LC3-Ⅱ和P62的影响。方法:在离体大鼠心肌细胞培养基础上制备心肌细胞缺血模型,随机分为正常对照组、缺血损伤组和EPO干预缺血损伤组。全自动生化分析仪测定培养液中血清肌酸激酶同功酶(CK-MB)、心肌肌钙蛋白I(c Tn I)的含量;免疫印迹方法检测心肌细胞中微管相关蛋白1轻链3-Ⅱ(LC3-Ⅱ)、P62的表达水平。结果:缺血损伤组心肌细胞培养基中CK-MB和c Tn I的含量及心肌细胞内LC3-Ⅱ、P62的表达水平较正常对照组明显增加(P <0. 01);经EPO干预后细胞培养基中CK-MB和c Tn I的含量及心肌细胞内LC3-Ⅱ、P62的表达水平较缺血损伤组明显降低(P <0. 01)。结论:EPO可通过降低大鼠心肌细胞自噬发挥心肌保护作用。

Objective： To observe the effect of erythropoietin （EPO） on autophagy of the ischemia myocardial cells. Methods： Rat myocardial cells were cultured and randomly divided into normal group （n=6）, ischemia injured group （n=6） and EPO-treated group （n=6）. Then the myocardial ischemia model was set up. After that, the concentrations of creatine kinase-MB （CK-MB） and cardiac troponin I （cTnI） were measured in each group. By Western blotting, the autophagy related protein microtubule associated protein 1 light chain 3 （LC3）-Ⅱand P62 were detected in myocardial cells of each group. Results： As compared with normal group, the concentrations of CK-MB and cTnI in culture medium, expression levels of LC3-Ⅱand P62 in cardiomyocytes were significantly increased than those in ischemia injured group. As compared with ischemia injured group, the concentrations of CK-MB and cTnI in culture medium, expression levels of LC3-Ⅱand P62 in cardiomyocytes were significantly decreased in EPO-treated group. Conclusion： EPO can play the role of myocardial protection by reducing the autophagy of rat cardiomyocytes.