红果型沙地云杉内生真菌多样性

Endophytic Fungus Diversity of Red Fruit Type Pice Mongolica

了解沙地云杉植物内生真菌生态多样性,为沙地云杉内生真菌的种属鉴别和资源的开发利用提供科学的依据,并为下一步深入研究内生真菌代谢产物提供理论基础。对内蒙古自治区克什克腾旗白音敖包自然保护区的沙地云杉进行调查,采用组织分离法对沙地云杉的不同组织部位进行内生真菌分离,以形态学和分子生物学相结合的方法进行鉴定,选取其中60株进行扩增、测序和系统发育分析。从系统发育树可见,菌株聚为2个大分支,分别为子囊菌亚门和半知菌亚门,10个小分支对应7个科分属于10个属12种,其中链格孢属(Alternaria)与青霉属(Penicillium)为优势菌属,红果型沙地云杉内生真菌菌种资源较丰富。

The objectives of this study were to understand the ecological diversity of the endophytic fungi in sandy spruce plants,to provide a scientific basis for the species identification and exploitation and utilization of endophytic fungi in sandy spruce,and to provide a theoretical basis for further study of endophytic fungal metabolites.An on-site investigation was carried out on the spruce occurring in sandy land in Baiyinobo Nature Reserve,Keshiketeng Banner,Inner Mongolia.Endophytic fungi were isolated from different tissues of Picea mongolica .Morphology and molecular biology of 60 strains were selected for amplification,sequencing and phylogenetic analysis.It was found from the phylogenetic tree that the strains were clustered into two major branches：Ascomycota and Candida albacares,respectively.There were seven branches belonging to 10 genera and 10 species belonging to 12 genera,of which Alternaria and Penicillium were the dominant genus.Red sandy spruce was rich in the resources of endophyte fungi species.