悬浮聚合法制备同型半胱氨酸分子印迹聚合物微球及其应用

Preparation of Homocysteine Molecularly Imprinted Polymer Microspheres with Suspension Polymerization and Its Application

以同型半胱氨酸(HCY)为模板,丙烯酰胺为功能单体,乙二醇二甲基丙烯酸酯为交联剂,聚乙烯醇为分散剂,偶氮二异丁腈为引发剂,甲苯为稀释剂,以800r·min^(-1)转速在55℃水性体系中采用悬浮聚合法制备了HCY分子印迹聚合物微球。制得的分子印迹聚合物微球大小均匀,最佳吸附时间为40min,最大表观吸附量为1.61mg·g^(-1),对结构类似的氨基酸具有良好的选择性。同型半胱氨酸的质量浓度在24mg·L^(-1)以内与其对应的吸光度呈线性关系,检出限(3s)为0.019mg·L^(-1)。方法用于家兔血液样品的分析,加标回收率为98.4%～109%,测定值的相对标准偏差(n=5)为0.75%～3.5%。

Homocysteine （HCY） molecularly imprinted polymer microspheres were synthesized by suspension polymerization using homocysteine as template, acrylamide as functional monomer, ethylene glycol two methyl acrylate （EGDMA） as crosslinking agent, polyvinyl alcohol （PVA） as dispersant, azobisisobutyronitrile （AIBN） as initiator and toluene as diluent in water system of 55 ℃ at the speed of 800 r · min^- 1. It was found that the prepared particles were uniform in size, the optimum adsorption time was 40 min, the maximum apparent adsorption capacity was 1.61 mg · g ^-1, and the selectivity to other amino acids which structure were similar to HCY was very good. Linear relationship between values of absorbance and mass concentration of homocysteine was obtained within 24 mg· L^- 1, with detection limit （3s） of 0.019 mg· L^-1. The proposed method was applied to the analysis of the sample of rabbit blood, giving values of recovery and RSDs （n = 5） in the ranges of 98.4%- 109% and 0.75% - 3.5% respectively.