结核分枝杆菌Rv1860T细胞表位预测及分析

Prediction and analysis of T cell epitopes of mycobacterium Rv1860 tuberculosis

对结核分枝杆菌Rv1860的T细胞表位进行预测及分析。从NCBI数据库得到Rv1860的蛋白质序列,运用Blastp分析Rv1860蛋白与人类蛋白的同源性,NetMHCⅡ预测分析Rv1860的CD4+T细胞抗原表位,SYFPEITHI、NetMHC、BIMAS与IEDB预测分析Rv1860的CD8+T细胞抗原表位。结果表明,Rv1860的CD4+T细胞抗原表位有24条强结合肽段,117条弱结合肽段;CD8+T细胞抗原表位有3条结合性较强的肽段。综合CD4+T细胞和CD8+T细胞抗原表位分析,得到1条优势抗原表位肽段为WLGTANNPV263—271,为进一步临床实验和结核病诊断以及疫苗的设计打下基础。

The T ceil epitope of Mycobacterium tuberculosis Rv1860 was predicted and analyzed. The protein sequence of Rv1860 was obtained from NCBI database, the homology of Rv1860 protein with human pro rein was analyzed by Blastp, the CD4^＋ T cell epitope of Rv1860 was predicted by NetMHCII, and the CD8^＋T cell epitope of Rv1860 was predicted by SYFPEITHI, NetMHC, BIMAS and IEDB. The results showed that the CD4^＋ T cell epitope of Rv1860 had 24 strong binding peptides and 117 weakly binding peptides; the CD8^＋ T cell epitope had three peptides with stronger binding. Based on the analysis of epitopes of CD4^＋ T cells and CD8^＋ T cells, one dominant epitope peptide was obtained as WLGTANNPV263-271, which laid a foundation for further clinical experiments and tuberculosis diagnosis and vaccine design.