急性氨氮暴露对大弹涂鱼炎性反应相关基因表达的影响

Effects of acute ammonia exposure on gene involved in inflammation of mudskipper

为研究急性氨氮胁迫对大弹涂鱼炎性反应相关基因表达的影响,实验挑选初始体质量为(15.14±0.05) g的健康大弹涂鱼幼鱼180尾,进行96 h的急性氨氮胁迫实验。结果显示,大弹涂鱼96 h氨氮半致死浓度为8.99 mg/L总氨氮(0.11 mg/L非离子氨,T-AN);氨氮胁迫后TNF基因的mRNA表达量分别于12和96 h时显著上调,96 h时表达量达到0 h时的2倍;IL-1基因的mRNA表达量12 h时显著上调,为0 h时表达量的6倍;氨氮胁迫后IL-6基因的mRNA表达量分别于12和96 h时显著上调,表达量达到0 h时的1.5倍;氨氮胁迫后IL-8基因的mRNA表达量在24 h时出现显著下调。研究表明,大弹涂鱼96 h氨氮半致死浓度为8.99 mg/L总氨氮;半致死浓度的氨氮胁迫48 h后,TNF、IL-1、IL-6和IL-8基因的mRNA表达量持续升高,推测过度炎性应激可能是导致鱼类氨中毒死亡的原因之一。

A study was carried out to test the effects of acute ammonia exposure on gene involved in inflammation of mudskipper Boleophthalmus pectinirostris. The mudskipper（15.14±0.05） g were randomly allocated to 2 groups（control group and exposure group） in triplicate for 96 h ammonia exposure. The 96 h lethal concentration50（LC50） of ammonia in mudskipper is 8.99 mg/L total ammonia nitrogen（TAN; 0.11 mg/L non-ionic ammonia）;Fish exposed to ammonia showed the mRNA expression of TNF increased significantly by 2-fold for 96 h; The mRNA expression of IL-1 increased significantly by 6-fold for 12 h; And the mRNA expression of IL-6 increased significantly by 1.5 fold for 12 h and 96 h; however, the mRNA expression of IL-8 decreased significantly for 24 h.This study indicated that the 96 h LC50 of ammonia in mudskipper is 8.99 mg/L TAN; The mRNA expression of TNF, IL-1, IL-6, IL-8 increased significantly after fish exposed to ammonia for 48 h; The inflammation is considered as one of major causes of fish ammonia poisoning.