HPLC-DAD同时测定养肝解毒丸中5种活性成分的含量

Simultaneous Determination of Five Kinds of Components in Yangganjiedu Pills by HPLC-DAD

目的:建立高效液相色谱法同时测定养肝解毒丸中龙胆苦苷、马钱苷、迷迭香酸、丹皮酚和五味子醇甲含量的方法。方法:采用Waters HSS C_(18)色谱柱;流动相:甲醇(A)-0. 5%的磷酸水溶液(B),梯度洗脱;柱温:30℃;流速:1. 0 m L·min^(-1);检测波长:马钱苷、五味子醇甲240 nm,龙胆苦苷、丹皮酚275 nm,迷迭香酸330 nm。结果:龙胆苦苷、马钱苷、迷迭香酸、丹皮酚和五味子醇甲的线性范围分别为5. 60～280、4. 56～228、2. 03～101. 5、6. 82～341、2. 18～109μg·m L^(-1)(r≥0. 999 3),5种成分的平均加样回收率(n=6)分别为99. 1%、98. 1%、98. 5%、96. 9%、97. 4%,RSD分别为0. 9%、0. 8%、1. 5%、1. 5%、1. 7%。结论:本法结果准确,重现性好,可用于养肝解毒丸的质量控制。

Objective： To establish an HPLC method for determination of gentiopicroside, loganin, rosmarinic acid, paeonol and schisandrol A in Huexuexiaozhong pills. Methods： A stable HPLC method was established and the sepa- ration was carried on a Waters Hss C18 column, the mobile phase consisted of methanol（A）-0.5% （w）phosphoric acid（B） in a gradient mode. The flow rate was 1.0 mL ·min- 1 and the column temperature was set at 30 ℃. The detection wavelength was set at 240 nm for loganin and schisandrol A, 275 nm for gentiopicroside and paeonol, 330 nm for roslnarinic acid, respectively. Result： The linear ranges of gentiopicroside, loganin, rosmarinic acid, paeonol and sehisandrol A were 5.60-280, 4.56-228, 2.03 -101.5, 6.82 -341 and 2. 18 -109 μg·mL-1（ r≥ 0. 999 3）, respectively. The average recovery were 99. 1% , 98. 1% , 98.5% , 96.9% and 97.4% , RSD were 0.9% , 0.8% , 1.5% , 1.5% and 1.7%. Conclusion： The method is accurate and reproducible, which can be used for the quality control of Yanggan- jiedu pills.