UV测定不同季节苗药加嘎吉给总酚酸的含量

UV Determination of Miao Medicine in Different Seasons and Ga to The Content of Total Phenolic Acids

目的:建立测定加嘎吉给中总酚含量的测定方法。方法:以没食子酸为对照品,采用分光光度法在259nm处测定加嘎吉给中多酚的含量。结果:没食子酸在0.003 2~0.016 0mg/mL范围内呈良好线性关系(y=1.389 3x-0.074 1,R2=0.9999),回收率的RSD为3.25%(n=6)。结论:该方法简便、准确,具有良好的精密度、稳定性、重复性,可用于加嘎吉给中总酚酸的含量测定。

Objective：To establish a method for the determination of total phenols in Agrimony.Methods：Using gallic acid as reference substance,the content of polyphenols in Agrimony was determined by spectrophotometry at 259 nm.The linearity of gallic acid in the range of 0.003 2~0.016 0 mg/mL was good（y=1.389 3 x-0.074 1,R2=0.999 9）.Results：The RSD of recovery was3.25%（n=6）.The method is simple and accurate,and has good precision,stability and reproducibility.Conclusion：It can be used for the determination of flavonoids and total phenols in Agrimony.