小儿宣肺止咳颗粒中多指标成分含量测定

Determination of Multi-marker Components in Xiaoer Xuanfeikezhi Granules

目的:建立小儿宣肺止咳颗粒中盐酸麻黄碱、盐酸伪麻黄碱、苦杏仁苷的含量测定方法。方法:采用Kromasil 100-5Phenyl(250mm×4.6mm,5μm)色谱柱,流速为1.0mL·min-1,以乙腈-0.1%磷酸水溶液(含0.04%三乙胺和0.02%二正丁胺)(1∶99)为流动相,检测波长为210nm,检测盐酸麻黄碱、盐酸伪麻黄碱含量;采用Agilent Hypersil ODS(250mm×4.0mm,5μm)色谱柱,流速为1.0mL·min-1,乙腈-0.1%磷酸水(6∶94)为流动相,检测波长为207nm,检测苦杏仁苷含量。结果:盐酸麻黄碱、盐酸伪麻黄碱、苦杏仁苷分别在31~993μg、29~933μg、0.09565~1.913μg质量范围内与峰面积积分线性关系良好,平均回收率分别为102.43%、101.60%、99.81%,RSD分别为1.27%、1.31%、1.20%。结论:高效液相色谱法准确、可靠、简便、迅速,适用于测定小儿宣肺止咳颗粒中盐酸麻黄碱、盐酸伪麻黄碱、苦杏仁苷的含量。

Objective：To establish the method for determination of ephedrine hydrochloride,ephedrine hydrochloride and amygdalin in Xiaoer xuanfeikezhi granules.Methods：The method of content detection of ephedrine hydrochloride,pseudoephedrine hydrochloride as follows,the column was eluted with Kromasil 100-5 Phenyl（250 mm×4.6 mm,5μm）at a flow rate of 1.0 mL·min-1 with acetonitrile-0.1% phosphoric acid（containing 0.04%triethylamine and 0.02%1∶99）as the mobile phase,the detection wavelength was 210 nm.The method of content detection of amygdalin as follows,the mobile phase was acetonitrile-0.1%phosphoric acid water（6∶94）at a flow rate of 1.0 mL·min-1 on an Agilent Hypersil ODS column（250 mm×4.0 mm,5μm）.Results：The linear relationship between the concentration of ephedrine hydrochloride,pseudoephedrine hydrochloride and amygdalin in the range of 31~993μg,29~933μg and 0.09565~1.913μg was good with the average recoveries of 102.43%,101.60%and 99.81%,（RSD 1.27%,1.31%,1.20%,respectively）.Conclusion：HPLC is accurate,reliable,simple and rapid.It is suitable for the determination of ephedrine hydrochloride and ephedrine hydrochloride in Xiaoer Xuanfei Zhike Granule.