基于高通量测序技术研究白甲鱼肠道微生物群落组成

Microbial Community in the Gut of Onychostoma sima Studied by High-throughput Sequencing

为研究白甲鱼肠道微生物菌群组成及其多样性.通过高通量测序技术研究白甲鱼肠道内容物菌群组成结构及多样性.结果表明:从36只健康白甲鱼肠道内容物样品中测得白甲鱼肠道中共有22个门,202个物种组成的微生物群.白甲鱼肠道细菌以梭杆菌门Fusobacteria (70. 51±3. 72)%、变形菌门Proteobacteria (22. 55±4. 21)%、螺旋菌门Spirochaetae (2. 84±2. 14)%、厚壁菌门Firmicutes (2. 61±1. 10)%和软壁菌门Tenericutes (1. 12±0. 88)%为主.梭杆菌门中Cetobacterium平均占有(70. 51±3. 72)%的OTUs,是最大的细菌属. Fusobacteria和Proteobacteria为白甲鱼肠道中的优势菌群.实验分析了白甲鱼肠道微生物菌群结构,发现白甲鱼肠道内存在复杂的微生物菌群,为进一步研究白甲鱼对营养物质的吸收利用提供了理论基础.

In order to study the composition and diversity of microbial communities in the gut of Onychostoma sima. The composition and diversity of gut content bacteria were investigated by the high-throughput sequencing. A total of 22 phylum and 202 genus bacterial phylotypes （ operational taxonomical units, OTUs） were identified in the gut contents samples from 36 O. sima. There are mainly Fusobacteria （70. 51 ±3.72）%, Proteobacteria （22.55 ± 4. 21 ）%, Spirochaetae （2. 84 ± 2. 14）%, Firmicutes （2.61 ± 1.10）%, Tenericutes （1.12 ±0. 88）%. The ave rage number of Cetobacterium, with （70. 51 ± 3.72） % of all OTUs, was the most diverse bacterial genus in Fuso- bacteria. Fusobacteria and Proteobacteria were the dominant groups. There were complex microbial communities in the gut of the O. sima. The bacterial community structure of O. sima was prelimina- rily evaluated. This provides a theoretical basis for the further study of the absorption and utilization of nutritious materials.