发菜基因组DNA提取方法比较与分子鉴定研究

Comparison and identification of genomic DNA extraction methods from Nostoc flagelliforme

目的以发菜为研究对象比较不同的提取DNA方法,筛选出了一种适合快速提取发菜DNA的方法。方法分别利用自主研发的硅珠DNA纯化技术、细菌试DNA提取试剂盒和植物DNA提取试剂盒提取发菜基因组DNA。结果本实验室开发的硅珠DNA提取纯化技术具有快速、无毒以及获得的发菜DNA纯度高等优势。通过聚合酶链式反应(PCR)扩增和测序得到16S rRNA基因,利用Blast软件进行序列比对,与发菜同源性为99%。结论本研究研制的发菜基因组DNA提取方法可用于发菜源性的分子鉴定。

Objective To select a method for the rapid extraction of Nostoc flagelliforme DNA by comparing different DNA extraction methods. Methods The self-developed silica bead DNA purification technology,the bacterial DNA extraction kit and the plant DNA extraction kit were used to extract the Nostoc flagelliforme genomic DNA,respectively. Results The technology of extraction and purification of silica bead DNA developed by our laboratory has the advantages of rapid,non-toxic and high purity of DNA obtained from Nostoc flagelliforme .The 16S rRNA gene was obtained by PCR amplification and sequencing,and the sequence alignment was conducted by Blast software,with the homology of 99% of the same as Nostoc flagllifome . Conclusion This method can be used for the molecular identification of Nostoc flagllifome.