一测多评法同时测定车前子配方颗粒中3种成分含量

Simultaneous determination of three constituents in Plantago Seed formula granules by QAMS

为实现对车前子配方颗粒多指标成分的质量评价,解决中药化学对照品分离难度大、单体性质不稳定、供应价格高等问题,建立了一测多评法同时测定车前子配方颗粒中的3种有效成分含量。以京尼平苷酸为内标物,建立与芦丁、毛蕊花糖苷的相对校正因子,测定其含量,同时采用外标法测定这3种成分的含量,比较两种方法的结果差异。结果表明:京尼平苷酸、芦丁、毛蕊花糖苷分别在0.804~8.040,0.172~1.720,0.158~1.580μg范围内线性关系良好(r≥0.999),平均加样回收率分别为102.52%,102.00%和101.86%,RSD值分别为1.64%,1.06%和1.07%。一测多评法所得结果与外标法差异不明显,具有较高的重复性、稳定性及可信度,可为车前子配方颗粒的质量评价提供参考依据。

In order to achieve the quality evaluation of the multi-index components of Plantago Seed Formula Granules and to solve the problem of the difficulty in separating the chemical reference substances of traditional Chinese medicine and the unstable supply price of monomer properties, the quantitative analysis of multi-components by single marker （QAMS） is established to simultaneously determine the content of three effective components in Plantago Seed formula granules.Geniposidic acid is used as the internal reference substance, and the relative correction factor of Rutin and Verbascoside is established respectively, measuring the content, and the three constituents are determined by the relative correction factor. At the same time, the content of the 3 components is determined by external standard method, and the difference between the results of the two methods is compared. Geniposidic acid, Rutin and Verbascoside show good linear relationships within the ranges of 0.804-8.040,0.172-1.720,0.158-1.580 μg（ r ≥0.999）,whose average recovery rate is 102.52%,102.00%,101.86%,and the RSD is 1.64%,1.06%,1.07% correspondingly. The results obtained by QAMS are close to the external standard method. The results turn out that it is no obviously difference between the two Methods and the quantitative analysis of multi-components by single marker （QAMS） is accurate and feasible, which could provide a reference for the quality evaluation of Plantago Seed formula granules.