CTGF、VEGF在卵巢癌组织中的表达水平及其对卵巢癌腹膜转移和淋巴结转移的影响

The expression of CTGF and VEGF in ovarian cancer tissue and their effects on peritoneal metastasis and lymph node metastasis of ovarian cancer

目的检测结缔组织生长因子(CTGF)和血管内皮生长因子(VEGF)在卵巢癌组织中的表达水平,探究CTGF和VEGF对卵巢癌腹膜转移和淋巴结转移的影响。方法收集42例卵巢癌手术患者的卵巢癌组织标本,采用免疫组化链酶菌抗生素蛋白-过氧化物酶(SP)法对卵巢癌组织标本进行检测,观察卵巢癌组织中CTGF和VEGF的表达水平及微血管密度(MVD)、淋巴管密度(LVD),并对卵巢癌腹膜转移和淋巴结转移的影响因素进行分析。结果不同腹膜转移情况、淋巴结转移情况和分化程度卵巢癌患者卵巢癌组织中CTGF阳性表达率比较,差异均有统计学意义(P﹤0.05);不同病理分期、临床分期、腹膜转移情况、淋巴结转移情况、分化程度卵巢癌患者卵巢癌组织中VEGF阳性表达率比较,差异均有统计学意义(P﹤0.05)。CTGF阳性组患者的MVD明显高于CTGF阴性组,VEGF阳性组患者的MVD明显高于VEGF阴性组(P﹤0.05);CTGF阳性组患者的LVD高于CTGF阴性组,VEGF阳性组患者的LVD高于VEGF阴性组,但差异均无统计学意义(P﹥0.05)。多无逐步Logistic回归分析结果显示:VEGF阳性表达是卵巢癌患者发生腹膜转移的独立危险因素(P﹤0.05);CTGF阳性表达和VEGF阳性表达均是卵巢癌患者发生淋巴结转移的独立危险因素(P﹤0.05)。结论 CTGF和VEGF可以促进卵巢癌组织中微血管和淋巴管的生成,进而促进卵巢癌发生腹膜转移和淋巴结转移。

Objective To observe the expression of connective tissue growth factor （CTGF） and vascular endothelia growth factor （VEGF） in ovarian cancer tissue and to investigate the effect of CTGF and VEGF on peritoneal metastasis and lymph node metastasis in ovarian cancer. Method The specimens of 42 patients with ovarian cancer underwent ovarian cancer surgery were collected. The expression levels of CTGF, VEGF, microvessel density （MVD） and lymphatic vessel density （LVD） in ovarian cancer tissues were observed by immunohistochemical streptavidin-peroxidase （SP） method, and the factors influencing peritoneal metastasis and lymph node metastasis of ovarian cancer were analyzed.Result The positive expression rates of CTGF in ovarian cancer tissues of ovarian cancer patients with different peritoneal metastasis, lymph node metastasis and differentiation degree was significantly different （P〈0.05）. There were significant differences in the positive expression rate of VEGF in ovarian cancer tissues of ovarian cancer patients with different pathological stages, clinical stages, peritoneal metastasis, lymph node metastasis and differentiation degree （P〈0.05）. MVD of CT- GF positive group was significantly higher than that of CTGF negative group, and MVD of VEGF positive group was sig- nificantly higher than that of VEGF negative group （P〈0.05）. The LVD of CTGF positive group was higher than that of CTGF negative group, and that of VEGF positive group was higher than that of CTGF negative group, but there was no significant difference （P〉0.05）. Multivariate Logistic regression analysis showed that the positive expression of VEGF was independent risk factors for peritoneal metastasis in ovarian cancer patients （P〈0.05）, and the positive expression of CTGF and VEGF were both independent risk factors for lymph node metastasis in ovarian cancer patients （P〈0.05）. Conclusion The expression of CTGF and VEGF promotes the formation of microvessels and lymphatic vessels in ovarian cancer tissues and promotes peritoneal metastasis and lymphatic metastasis in ovarian cancer.