CorTector^(TM) SX100:一款桌面式荧光相关光谱仪的原理和应用

CorTector^(TM) SX100:Principles and Applications of a Bench-top Fluorescence Correlation Spectrometer

荧光相关光谱检测技术具有超灵敏(单分子)、快速(数秒至数分钟)和多功能(检测分子浓度、大小和相互作用)等技术优点,且无需反应物分离,因此有潜力成为一种新型均相、高敏荧光免疫检测技术,适用于在溶液中或单个活细胞内检测生物分子特性.本文首先介绍荧光相关光谱检测技术的原理和研究进展,然后结合项目团队自主研发的目前全球唯一一款可靠、易使用的桌面式荧光相关光谱仪,进一步探讨荧光相关光谱检测技术的具体实现和潜在应用.

Fluorescence correlation spectroscopy （FCS） technologies have the advantage of ultrasensitivity （single molecule）, fast speed （seconds and minutes measurement time）, and multifunctionality （simultaneously analyzing molecular concentration, size and interaction）.Thus, FCS technologies have the potential of being a novel fluorescence immunoassay suitable for high-sensitivity analysis of homogeneous sample solution. This article introduces the principles and applications of FCS technologies based on the reliable, easy-to-use, bench-top fluorescence correlation spectrometer developed by the project team.