长链非编码RNA Linc00152对肠癌细胞增殖迁移能力的影响

Effects of long non-coding RNA Linc00152 on proliferation and migration of colorectal cancer cells

目的探讨长链非编码RNA (lncRNA) Linc00152在肠癌中的表达及其对肠癌细胞增殖迁移能力的影响。方法合成针对Linc00152的小干扰RNA (si-00152)及无关序列si-NC,采用脂质体转染法将小干扰RNA si-00152及si-NC转染至人肠癌细胞株LOVO,si-00152_LOVO和siNC_LOVO。采用实时荧光定量PCR (real-time PCR)法检测si-00152下调前后LOVO细胞中Linc00152表达情况;采用MTT实验、划痕实验检测下调Linc00152前后细胞增殖和迁移能力变化。结果 LOVO、si-NC_LOVO和si-00152_LOVO细胞株中Linc00152表达水平分别为(8.23±1.02)、(7.98±1.34)和(2.78±1.06),差异有统计学意义(P<0.05),小干扰RNA可成功下调LOVO细胞株中Linc00152的表达水平;LOVO细胞培养48 h、72 h和96 h后吸光度A值分别为(0.50±0.08)、(0.87±0.09)和(1.54±0.11);si-NC_LOVO细胞分别为(0.44±0.10)、(0.79±0.08)和(1.37±0.12);si-0015_LOVO分别为(0.49±0.09)、(0.66±0.07)和(0.88±0.10)。si-00152_LOVO细胞96 h吸光度A值明显低于LOVO和si-NC_LOVO细胞,差异均有统计学意义(P<0.05);小干扰RNA下调LOVO细胞Linc00152表达后LOVO细胞迁移能力显著降低。结论 si-00152可显著下调肠癌细胞株LOVO中Linc00152表达水平,下调Linc00152表达可抑制肠癌细胞株LOVO的增殖和迁移能力。

Objective To evaluate the investigate the expression of long noncoding RNA （lncRNA） Linc00152 in colorectal cancer and its effect on the proliferation and migration of colorectal cancer ceils. Methods The small interference RNA for linc00152 （si-00152） and non-correlation siRNA （si-NC） was synthesized and transfected into human intestinal cancer cell lines LOVO, si-00152_LOVO and siNC LOVO by lipofection assay. Linc00152 expression in LOVO cells before and after down-regulation of si-00152 were detected by real-time fluorescent quantitative PCR （real-time PCR）. The changes of cell proliferation and migration ability before and after down-regulation of Linc00152 were detected by MMT test and wound healing assay. Results The expression level of lncRNA linc00152 in cell lines for LOVO, si-NC_LOVO and si-00152_LOVO were （8.23±1.02）, （7.98±1.34） and （2.78±1.06）, respectively, with statistically significant differences （P〈0.05）. Small interfering RNA can successfully decrease the expression of Linc00152 in LOVO cell lines. The absorbance A values of LOVO cells cultured at 48 h, 72 h and 96 h were （0.50±0.08）, （0.87±0.09） and （1.54±0.11）; （0.44±0.10）, （0.79±0.08） and （1.37±0.12） for si-NC_LOVO cell; （0.49±0.09）, （0.66±0.0） and （0.88±0.10） with si-0015_LOVO cells. The absorbance （A） value of si-00152_LOVO cells at 96 h was significantly lower than that of LOVO and si-NC_LOVO cells, with statistically significant difference （P〈0.05）. Wound healing assay showed that the migration ability was decreased after inhibiting linc00152 expression in LOVO cells. Conclusion Si-00152 can significantly down-regulate the expression of Linc00152 in LOVO, and the Linc00152 expression can inhibit the proliferation and migration of colorectal cancer cell line LOVO.