响应面法对亚临界水提取茶枯饼中茶皂素的优化研究

Study on Optimization of Subcritical Water Extraction Tea Saponin from Tea Cake by Response Surface Methodology

研究亚临界水提取法提取茶枯饼中茶皂素的工艺条件,以茶皂素提取率为指标,在单因素试验的基础上利用Box-Behnken进行响应面优化。建立各因素与响应值之间的数学模型,确定亚临界水提取法的可行性及最佳工艺条件。结果表明,亚临界提取最佳工艺条件为:反应温度110℃,时间24 min,料液比1︰30 g/mL,在此条件下,茶皂素提取率为27.72%,与预测值27.35%的吻合度达98.66%。结合扫描电镜结果,得出亚临界水提取法是一种高效、经济、绿色环保的茶皂素提取方法。

The technological process condition of extracting tea saponin from tea dried cakes by subcritical water extraction method was studied. With the extraction rate of tea saponin as the index, box-behnken was used to optimize the response surface on the basis of single-factor experiment. A mathematical model was established to determine the feasibility and optimum technological condition of subcritical water extraction. The results showed that the optimum condition for subcritical extraction was as follows： reaction temperature 110 ℃, time 24 min, and ratio of solid to liquid 1︰30 g/mL. Under this condition, the extraction rate of tea saponin was 27.72%, which was 98.66% in accordance with the predicted value of 27.35%. Combined with the results of scanning electron microscopy, it was concluded that subcritical water extraction was an efficient, economical and environmentally friendly method for extracting tea saponin.