鹿角盘成分提取及其工艺的优化

Extraction of Deer Hard Antler Button Powder and Optimization of Its Extraction Procedure

摸索鹿角盘提取条件,优化提取工艺。利用正交试验考察水提法和酶解法对鹿角盘提取率的影响。水提法:料液比1︰25 (g/mL), 100℃煎煮6 h,提取率为28.11%。单酶酶解法:胃蛋白酶酶解工艺为料液比1︰30 (g/mL),pH 1.8,加酶量4%,酶解温度37℃,酶解4 h,提取率为22.39%;胰蛋白酶酶解工艺为料液比1︰20 (g/mL), pH 8.0,加酶量4%,酶解温度55℃,酶解6 h,提取率为20.99%。复合酶解酶解工艺:先胃蛋白酶,处理条件为料液比1︰20 (g/mL),pH 1.8,加酶量1%,酶解温度37℃,酶解12 h;后胰蛋白酶,处理条件为料液比1︰20 (g/mL), pH 8.0,加酶量0.4%,酶解温度37℃,酶解2 h,提取率可达31.84%。按照复合酶解工艺,对水提法最佳条件下提取后的鹿角霜进行复合酶解,提取率仅为1.33%。利用水提和酶解的方法筛选出鹿角盘的最优提取条件,制备工艺合理。由结果看出,两个方法中得到的提取率差异不明显。复合酶解得到的提取物种类和含量多于水提法的种类和含量。

To explore the extraction conditions of deer hard antler button（DHAB）, optimize the extraction procedures. Effects of water extraction method and enzymatic digestion methods on the extraction rates of DHAB was studied by using orthogonal tests. Using the water extraction method, solid to liquid ratio was 1︰25（g/m L）, extraction temperature 100 ℃, extraction 6 h, the highest extraction rate of 28.11% was obtained. Single enzyme digestion to the optimum enzymolysis procedures of pepsin： solid to liquid ratio was 1︰30（g/m L）, pH 1.8, enzymolysis 4 h, quantity of enzyme loading 4%, digestion temperature 37 ℃, the extraction rate was 22.38%; The optimum enzymolysis procedures of trypsin： solid to liquid ratio was 1︰20（g/m L）,pH 8, enzymolysis 6 h, quantity of enzyme loading 4%, digestion temperature 55 ℃, extraction rate was 20.99%; Combined enzyme digestion process： solid to liquid ratio of pepsin was 1︰20（g/m L）, pH 1.8, enzyme loading 1%, digestion temperature 37 ℃, enzymolysis 12 h; After, solid to liquid ratio of trypsin was 1︰20（g/m L）, pH 8.0, quantity of enzyme loading 0.4%, digestion temperature 37 ℃, enzymolysis 2 h. The extraction rate was 31.84%. DHAB extracted under the best conditions of water extraction combined enzymolysis, the extraction rate was only 1.33%. Conclusion： the optimal extraction conditions of the DHAB were selected for water extraction and enzymatic digestion methods, and the extraction process was simple. Based on the results obtained by using these two methods, extraction rates were not significantly different. The specie and content of the extract obtained by the combined enzymolysis was more than that of the water extraction method.