摘要
建立藏王宝胶囊中红景天苷的含量测定方法。采用高效液相色谱法,以十八烷基键合硅胶为填料的Swell Chromplus C_(18) (250 mm×4.6 mm, 5μm)为色谱柱,以0.02 mol/L乙酸钠溶液-甲醇(8︰2)为流动相,流速为1.0 mL/min,检测波长为220 nm。红景天苷在0.01~2.84μg浓度范围内呈现良好的线性关系,该方法的MDL为0.013 8μg/kg,精密度N为6组、RSD为1.01%;稳定性N为6组、RSD为1.09%;重复性N为6组、RSD为0.48%;加样回收率N为9组、RSD为2.14%,藏王宝胶囊中红景天苷的平均质量含量为0.005 4 mg/kg。方法稳定可靠,切实可行,操作简便,结果准确,可用于藏王宝胶囊的质量控制。
To establish the method to determine the content of salidroside in healthy food Zangwangbao capsules. HPLC was used with swell chromplus C(18)(250 mm×4.6 mm, 5 m) as analysis column, 0.02 mol/L sodium acetate solution-methanol(8︰2) as the mobile phase. The flow rate was 1.0 mL/min, and the detection wavelength was set at 220 nm. A good linear relationship for salidroside obtained over the range of 0.01-2.84 μg. The method of MDL was 0.013 8 g/kg. N and RSD of the precision of the method was 6 and 1.01%; N and RSD of stability was 6 and 1.09%; N and RSD of the repeatability was 6 and 0.48%; N and RSD of the average recovery was 9 and 2.14%. The average content of salidroside was 0.005 4 mg/kg in Zangwangbao capsules. The method was stable and reliable, with convenient operation and accurate, and it could be used for quality control of Zangwangbao capsules.
作者
冯慧
赵娅
张静
赵永强
赖先荣
FENG Hui;ZHAO Ya;ZHANG ding;ZHAO Yongqiang;LAI Xianrong(Chengdu University of Traditional Chinese Medicine,Chengdu 611137;Tibet Yutuo Health Products Co.,Ltd.,Lasa 850000)
出处
《食品工业》
CAS
北大核心
2018年第11期322-325,共4页
The Food Industry
基金
国家自然科学基金青年科学基金项目(81203000)