人参皂苷Rg3对大鼠颈动脉球囊损伤后NF-κBp65及相关炎性因子表达的影响

Effect of Ginsenoside Rg3 on NF-κBp65 and Its Related Inflammatory Factors after Carotid Balloon Injury in Rats

目的研究人参皂苷Rg3对大鼠颈动脉球囊损伤后核因子(NF)-κBp65及相关炎症因子表达的影响。方法成年健康雄性SD大鼠40只,将其随机分成4组:假手术组,模型组,人参皂苷Rg3 5 mg·kg^(-1),人参皂苷Rg3 10 mg·kg^(-1)。用2. 0mm×12 mm球囊建立大鼠颈动脉损伤模型,次日灌胃给药,连续14 d;利用苏木精-伊红(HE)染色观察损伤血管组织形态学的改变;利用Western blot法检测损伤血管组织中NF-κBp65蛋白的表达水平;利用酶联免疫吸附法(ELISA)和Real-Time PCR法检测损伤血管组织中白细胞介素(IL)-1β和IL-6及肿瘤坏死因子-α(TNF-α)的表达。结果模型组大鼠血管新生内膜较假手术组明显增厚(P <0. 01),损伤血管NF-κBp65的表达明显增加(P <0. 01),IL-1β、IL-6及TNF-α的表达水平明显上升(P <0. 01);与模型组相比,人参皂苷Rg3(5、10 mg·kg^(-1))损伤血管内膜增生明显减轻(P <0. 01),NF-κBp65的表达明显减少(P <0. 01),IL-1β、IL-6及TNF-α的表达水平也明显下降(P <0. 01)。结论人参皂苷Rg3能抑制大鼠颈动脉球囊损伤后血管内膜增生及炎性反应,可能与其抑制转录因子NF-κB的表达水平有关。

OBJECTIVE To study the effects of ginsenoside Rg3 on NF-κBp65 and its related inflammatory factors after carotid balloon injury in rats. METHODS Sprague Dawley （SD） rats （n = 40） were divided into 4 groups randomly： sham operationgroup, model group, ginsenoside Rg3 5 mg·kg^-1 and ginsenoside Rg3 10 mg·kg^-1. 2.0 mm × 12 mm Ballon catheters were used to establish the carotid artery intima injury model. On next day after modeling, all animals in model group were administered intragastrically with relative saline and different concentration of ginsenoside Rg3 for 14 d. After 14 d, the morphological changes of the injured arteries were observed by HE staining. The expression of NF-κBp65 in vascular tissue was detected by Western blot ; Tumor necrosis factor- α（TNF-α） , interleukin-1β（IL-1β） and IL-6 were detected by RT-PCR and Elisa. RESULTS Compared with the sham-operation group, the neointimal in model group was significantly thicker（ P 〈 0. 01 ）. while the expression levels of IL-1β, IL-6, TNF-α and NF-κBp65 were increased in model group（ P 〈 0. 01 ）. The vascular intimal hyperplasia was alleviated distinctly（ P 〈 0.01 ） and the protein expression of NF-KBp65 in vascular tissue was significantly decreased（ P 〈 0.01 ） compared with model group, the expression level of IL-1β, IL-6 and TNF-α was significantly lower in intervention group （P 〈 0.01 ）. CONCLUSION Ginsenoside Rg3 could reduce the vascular neointimal hyperplasia and inflammation induced by balloon injury, which may be related to its inhibitory role of the transcription factor NF-κB signaling pathway.