天然止咳化痰乳剂对人工诱导兔子病理模型抗损伤的观察

Observation of natural antitussive and expectorant emulsion on antioxidative injury of rabbit

为了验证止咳化痰乳剂的临床治疗效果。将15只健康的家兔随机分为A、B、C 3组,每组5只。A组为正常对照组,每天给予正常饲喂; B组模型组用97 mg/m^3氨水喷雾刺激,连续刺激9 d。C组治疗组。每次开始试验前1 h以0. 5 ml∕只的剂量灌服止咳化痰药,其余条件同模型组。第9天每只兔子心脏采血5 mL进行血相和肝肾生化分析,然后将A、B、C 3组家兔处死,手术切除全肺,制作肺组织的病理切片,H. E.染色。结果显示,97 mg/m^3氨气浓度刺激兔子,血氨浓度持续增高。模型组兔子白细胞、淋巴细胞、单核细胞、嗜中性粒细胞、ALT、BUN随着氨气刺激比对照组分别提高58. 7%、71. 7%、50. 1%、116. 7%、106. 5%、91. 6%,经止咳化痰乳剂治疗后白细胞、淋巴细胞、单核细胞、嗜中性粒细胞、ALT、BUN降低31.%、36. 7%、50. 1%、29. 2%、34. 7%、17. 39%。病理切片显示,模型组肺脏可见肺间质有大量红细胞、炎症细胞浸润,腔内有大量红细胞渗出,部分肺泡腔变小且肺泡壁变厚甚至实变;治疗组的肺损伤明显好转。

In order to verify the clinical efficacy of Antitussive and expectorant emulsion,15 rabbits were randomly divided intoA, B and C groups With 5 rabbits in each group. Group A was normal control without ammonia stimulation. Group B was stimulatedwith 97 mg / m^3ammonia conce 2 hners, 2 times one day） for nine days, which was used to establish a model of lung injury. GroupC was stimulated with ammonia at the same concentration, but every rabbit was fed with medicine at the dose of 0. 5ml before the ex-periment. Other conditions were the same. On the ninth day, 5ml blood were collected and liver and kidney tissues were alsol sam-pled for biochemical analysis, and then all the rabbits in group A, B, C, lung sections were couected for pathological analysis HEstaining. The results showed that the ammonia stimulation at the concentration of 97 mg / m^3increased rabbit blood ammonia concentra-tion continuously. The white blood cells, lymphocytes, monocytes, neutrophils, ALT, and BUN in rabbits with 58. 7%, 71. 7%,ammonia stimulation increased by 50. 1%, 116. 7%, 106. 5% and 91. 6% when compared with those in the control group. respec-tively The white blood cells, lymphocytes, monocytes, neutrophils, ALT, and BUN were decreased about 31% after treatment36. 7%, 50. 1%, 29. 2%, 34. 7%, 17. 39% with cough and phlegm emulsion. Pathologcal analysis showed that the lung interstiti-um has a large number of red blood cells, inflammatory cell infiltration in nodel graup, and alveolar cavity has a large number of redblood cells exudation, and became smaller, The alveolar wall was thickened and consolidated. However, lung injury in the treatmentgroup was significantly improved.