甘油对红曲霉CG-6莫纳可林K合成及相关基因表达的影响

Effects of Glycerol on Monascus CG-6 Monacolin K Synthesis and Related Gene Expression

研究甘油对红曲霉CG-6莫纳可林K合成及相关基因表达的影响,采用高效液相色谱和荧光定量PCR法测定莫纳可林K产量及相关基因表达量的变化。结果表明:甘油浓度在0～12%时,随其浓度增加,胞内和胞外总莫纳可林K积累量呈先增加后减少趋势,甘油浓度为8%时,胞内和胞外总莫纳可林K积累量均达到最大,分别为9 474. 4、7 005.8μg/(g干菌重);甘油浓度为8%,随着培养时间延长,莫纳可林K合成相关基因mokA、mokD、mokE、mokG、mokH、mokI表达量较对照组逐渐升高,mokB、mokC表达量变化不大,mokF表达量逐渐下降;甘油浓度大于8%培养9 d时,mokB、mokC、mokD、mokE、mokF、mokG、mokH出现下调趋势。实验结果为研究甘油等碳源对红曲霉次级代谢产物合成及相关基因表达影响提供了参考。

In this paper, effects of glycerol on the synthesis and related genes expression of Monascus CG-6 Monacolin K were studied. The yields of Monacolin K and related genes expression were determined by high performance liquid chromatography and real-time PCR. The results showed that the accumulation of intracellular and extracellular Monacolin K increased first and then decreased with the increase of gly- cerol concentration in the range of 0-12% after 9 days of culture. When the concentration of glycerol was 8%, the accumulation of intracellular and extracellular Monacolin K reached the maximum, which was 9 474.4 μg/g and 7 005.8 μg/g dry weight. With the prolongation of culture time, the expression of mokA, mokD, mokE, mokG, mokH, and mokI of Monacolin K synthesis genes increased gradually compared with the control group, while the expression of mokB and mokC did not change much, and the expression of mokF decreased gradually; When glycerol concentration was greater than 8% for 9 days, mokB, mokC, mokD, mokE, mokF, mokG , and mokH showed a downward trend. The results provide a reference for studying the effects of carbon sources such as glycerol on the synthesis of secondary metabolites and the expression of related genes in Monascus .