BMP-2对大鼠延迟再植牙牙周膜愈合影响的实验研究

Effect of bone morphogenetic protein-2 (BMP-2) on periodontal regeneration after delayed replantation of avulsed tooth in rat

目的探讨骨形态发生蛋白2(bone morphogenetic protein-2,BMP-2)对大鼠牙脱位后延迟再植牙周膜愈合的影响。方法将16只体重250～300g的雄性Wistar大鼠随机分为对照组、实验组各8只,2组均将脱位牙自然干燥1h,体外行根充、玻璃离子根尖封闭。实验组牙根表面涂抹BMP-2糊剂后再植,对照组牙根则不涂抹。术后8周取出含牙骨块,进行脱钙、切片及HE染色,对牙根的愈合情况进行组织形态学评价。结果实验组与对照组牙骨质愈合、牙根替代性吸收和炎症性吸收差异均无统计学意义(P>0.05)。结论 BMP-2可在一定程度上有利于牙骨质愈合,但尚不能认为其能有效抑制脱位牙延迟再植的牙根吸收。

Objective To investigate the effect of bone morphogenetic protein-2（BMP-2）on periodontal regeneration after delayed replantation of avulsed tooth in rat.Methods Sixteen male Wistar rats weighed 250 to 300 g were randomly assigned to a control group（n =8）and experimental group（n=8）.The teeth were extracted and air-dried for 1 hour.The root canals were endodontically treated and the root apexes were sealed with glass ionomer.In experimental group,BMP-2 paste is applied to root surface of each tooth and replanted in the original socket.Teeth in control group is replanted without any surface treatment.After 8 weeks of replantation,the bone segment from the replantation site is obtained,decalcified and evaluated histologically.Results There was no statistically significant difference regarding the cementum regeneration,replacement root resorption and inflammatory root resorption in both groups（P〉0.05）.Conclusion The results indicate that the application of BMP-2 may promote cementum regeneration and inhibit the inflammatory root resorption.However,the inhibitory effect was not significant in the delayed replantation of avulsed teeth.