急性肺栓塞患者血清miRNAs标志物检测及miR-134的临床价值

Detection of serum miRNAs markers and clinical value of miR-134 in patients with acute pulmonary embolism

目的探讨急性肺栓塞患者血清miRNAs标志物检测及miR-134的临床价值。方法收集50例急性肺栓塞患者及25例对照者的血清,应用miRNA表达谱芯片进行筛选,并采用实时荧光定量聚合酶链反应进行验证,检测急性肺栓塞患者血清miRNAs的表达,分析miR-134标志物与Wells评分之间的关系及其诊断价值。结果血清miRNA表达谱分析发现,在6例急性肺栓塞和6例健康对照者中筛选出10个miRNA(miR-134、miR-197、miR-126、miR-191、miR-122、miR-370、miR-155、miR-133a、miR-375、miR-208b)存在表达差异(t分别=14.17、12.26、11.13、10.02、9.34、17.26、15.22、7.57、3.62、7.24,P均<0.05)。进一步在50例急性肺栓塞患者中做血清miRNAs验证,结果显示:miR-134、miR-197和miR-126在急性肺栓塞患者血清中表达水平较对照组高,而miR-208b表达水平较对照组低(t分别=7.65、6.23、4.36、-5.24,P均<0.05)。血清miR-134相对表达量与Wells评分呈明显正相关(r=0.32,P<0.05)。当miR-134相对表达量取5.6时,miR-134对急性肺栓塞的诊断灵敏度为72.00%(36/50),特异度为82.00%(41/50),曲线下面积为0.86。结论 miR-134、miR-197、miR-126和miR-208b均可作为急性肺栓塞患者的血清miRNAs标志物,且miR-134在诊断和预测急性肺栓塞风险中具有较高的临床价值。

Objective To detect the serum miRNAs markers and explore the clinical value of miR-134 in patients with acute pulmonary embolism. Methods The serum samples of fifty cases of acute pulmonary embolism and 25 healthy controls were collected. The serum miRNAs markers were screened by miRNA expression profile chip,and the real-time fluorescent quantitative polymerase chain reaction was performed to verify the miRNAs markers. The relationship between miR-134 markers and Wells score and its diagnostic value were analyzed. Results The expression profiles of serum microRNAs in 6 patients with acute pulmonary embolism and 6 healthy controls showed that 10 kinds of microRNAs（miR-134,miR-197,miR-126,miR-191,miR-122,miR-370,miR-155,miR-133a,miR-375 and miR-208b）were screened-out and differentially expressed（t=14.17,12.26,11.13,10.02,9.34,17.26,15.22,7.57,3.62,7.24,P〈0.05）. The expression levels of miR-134,miR-197 and miR-126 in the serum of patients with acute pulmonary embolism were higher than those of the healthy controls,while the expression level of miR-208b was lower（t=7.65,6.23,4.36,-5.24,P〈0.05）. The serum miR-134 was positively related to the Wells score（r=0.32,P〈0.05）. When the cut-off value of miR-134 is 5.6,the diagnostic sensitivity of miR-134 for acute pulmonary embolism was 72.00%（36/50）,the specificity was 82.00%（41/50）,and the area under the curve was 0.86. Conclusion All of miR-134,miR-197,miR-126 and miR-208b can be used as markers of serum microRNAs on patients with acute pulmonary embolism,and miR-134 has high clinical value in the diagnosis and prediction of the risk of acute pulmonary embolism.