咬合功能低下对正畸中牙龈组织CTGF表达的影响

A Study on the Effects of Occlusal Function on CTGF Expression in Orthodontics

目的动物实验研究CTGF在正畸治疗前后咬合功能低下牙牙龈组织中表达的变化,初步探索牙龈组织的改建机制及干预措施的有效性.方法将12周龄雄性Sprague-Dawley大鼠72只,体重250~300 g左右,随机等量分为空白对照组(A)、健康牙正畸组(B)、咬合功能低下组(C)、咬合功能低下牙正畸组(D)、咬合力干预组(E)、咬合力干预正畸组(F),每组各12只,各组动物分别按要求建模处理后第1周、2周、4周、6周,取动物模型上颌牙龈组织进行荧光定量检测,所得结果进行对比研究.结果(1)咬合功能低下组牙龈组织HE染色结果呈现萎缩样变,提示成功建立咬合功能低下SD大鼠模型.(2)牙龈组织CTGF的荧光定量结果:第6周:B组高于D组和F组(P<0.05).结论(1)咬合功能低下组CTGF的表达随时间变化较正常对照组低,牙龈组织易发生萎缩;(2)咬合力恢复是否是有效干预措施还有待进一步研究.

Objective To investigate CTGF changes in the expression of the gum tissue before and after orthodontic treatment, and to preliminarily explore the modification mechanism of gingival tissue and the effectiveness of the intervention measures. Methods 72 male Sprague-Dawley rats of 12-week-old, weight about 250 g-300 g, randomly divided into 6 groups： blank control group（A）, healthy teeth orthodontic group（B）, low functional group（C）, low occlusal function teeth orthodontic group（D）, combined intervention group（E）, bite orthodontic intervention group（F）. The results of the study were compared at 1 w, 2 w, 4 w,6 w.Results（1）HE staining results showed the atrophy of the gingival tissue, which suggested that occlusal hypofunction SD rats model were successfully established.（2） Fluorescence quantitative results of CTGF in gingival tissue： 6 w： group B was higher than group D and group F（P〈0.05）. Conclusion（1） the expression of CTGF in the low occlusion group was lower than that of the normal control group with time, and the gum tissue was vulnerable to atrophy.（2）It remains to be further studied whether the bite force recovery is effective.